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Shivhare R, Asif MH, Lata C. Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet. Plant Mol Biol. 2020;103(6):639-652doi: 10.1007/s11103-020-01015-w.
Shivhare, R., Asif, M. H., & Lata, C. (2020). Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet. Plant molecular biology, 103(6), 639-652. https://doi.org/10.1007/s11103-020-01015-w
Shivhare, Radha, et al. "Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet." Plant molecular biology vol. 103,6 (2020): 639-652. doi: https://doi.org/10.1007/s11103-020-01015-w
Shivhare R, Asif MH, Lata C. Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet. Plant Mol Biol. 2020 Aug;103(6):639-652. doi: 10.1007/s11103-020-01015-w. Epub 2020 May 19. PMID: 32430635.
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Plant Mol Biol. 2020 Aug;103(6):639-652. doi: 10.1007/s11103-020-01015-w. Epub 2020 May 19.

Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet.

Plant molecular biology

Radha Shivhare, Mehar H Asif, Charu Lata

Affiliations

  1. CSIR- National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India.
  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
  3. CSIR- National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India. [email protected].
  4. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. [email protected].
  5. CSIR-National Institute of Science Communication and Information Resources, 14 Satsang Vihar Marg, New Delhi, 110067, India. [email protected].

PMID: 32430635 DOI: 10.1007/s11103-020-01015-w

Abstract

Pearl millet is a widely cultivated grain and forage crop in areas frequented with hot and dry weather, and high temperature. Being cultivated in arid and semi-arid regions, the crop often encounters intermittent water stress either at early stages of development or flowering stage or both. However, its asynchronous tillering behavior and fast growth rate helps recovering from drought stress at vegetative stages while there is no such reprieve under terminal stress (flowering through grain filling). At present, the molecular basis of terminal drought tolerance of certain pearl millet genotypes remains elusive. In this study, a comparative transcriptome analysis has been performed at both vegetative and flowering stages of a terminal drought tolerant genotype, PRLT2/89-33, subjected to drought stress. The gene expression profiling analysis showed that PRLT2/89-33 has an inherent ability to sense drought at both developmental stages. Gene Ontology (GO) and MapMan pathway analyses underlined that flavanoid pathway, lignin biosynthesis, phenyl propanoid pathway, pigment biosynthesis, and other secondary metabolite pathways were enriched in control and drought stressed PRLT2/89-33 at flowering stage than at the vegetative stage. To our knowledge, this is the first report of comparative transcriptome analysis under drought stress at two different developmental stages which can facilitate fastidious discovery of drought tolerant genes leading to improved yield in pearl millet and other related crops.

Keywords: Comparative; Gene expression; Reproductive; Terminal drought; Transcriptome; Vegetative

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