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Glassop D, Stiller J, Bonnett GD, et al. An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression. Funct Plant Biol. 2017;44(8):795-808doi: 10.1071/FP17073.
Glassop, D., Stiller, J., Bonnett, G. D., Grof, C. P. L., & Rae, A. L. (2017). An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression. Functional plant biology : FPB, 44(8), 795-808. https://doi.org/10.1071/FP17073
Glassop, Donna, et al. "An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression." Functional plant biology : FPB vol. 44,8 (2017): 795-808. doi: https://doi.org/10.1071/FP17073
Glassop D, Stiller J, Bonnett GD, Grof CPL, Rae AL. An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression. Funct Plant Biol. 2017 Jul;44(8):795-808. doi: 10.1071/FP17073. PMID: 32480608.
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Funct Plant Biol. 2017 Jul;44(8):795-808. doi: 10.1071/FP17073.

An analysis of the role of the ShSUT1 sucrose transporter in sugarcane using RNAi suppression.

Functional plant biology : FPB

Donna Glassop, Jiri Stiller, Graham D Bonnett, Christopher P L Grof, Anne L Rae

Affiliations

  1. CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, Qld 4067, Australia.
  2. School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.

PMID: 32480608 DOI: 10.1071/FP17073

Abstract

The role of ShSUT1 in sucrose mobilisation and storage in sugarcane was investigated by employing RNAi technology to reduce the expression of this gene. Transcript profiling in non-transformed plants showed an alignment between expression and sucrose concentration, with strongest expression in source leaves and increasing expression through the daylight period of a diurnal cycle. Five transgenic plant lines were produced with reduced ShSUT1 expression ranging from 52 to 92% lower than control plants. Differential suppression of ShSUT1 sequence variants in the highly polyploid sugarcane genome were also investigated. Amplicon sequencing of the ShSUT1 variants within the transgenic lines and controls showed no preferential suppression with only minor differences in the proportional expression of the variants. A range of altered sugar, fibre and moisture contents were measured in mature leaf and internode samples, but no phenotype was consistently exhibited by all five transgenic lines. Phenotypes observed indicate that ShSUT1 does not play a direct role in phloem loading. ShSUT1 is likely involved with retrieving sucrose from intercellular spaces for transport and storage.

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