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Biomater Sci. 2013 Feb 03;1(2):224-230. doi: 10.1039/c2bm00114d. Epub 2012 Nov 05.

Bio-ink for on-demand printing of living cells.

Biomaterials science

Cameron J Ferris, Kerry J Gilmore, Stephen Beirne, Donald McCallum, Gordon G Wallace, Marc In Het Panhuis

Affiliations

  1. Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, University of Wollongong, Wollongong, NSW 2522, Australia. [email protected].

PMID: 32481802 DOI: 10.1039/c2bm00114d

Abstract

Drop-on-demand bioprinting allows the controlled placement of living cells, and will benefit research in the fields of tissue engineering, drug screening and toxicology. We show that a bio-ink based on a novel microgel suspension in a surfactant-containing tissue culture medium can be used to reproducibly print several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods. The bio-ink maintains a stable cell suspension, preventing the settling and aggregation of cells that usually impedes cell printing, whilst meeting the stringent fluid property requirements needed to enable printing even from many-nozzle commercial inkjet print heads. This innovation in printing technology may pave the way for the biofabrication of multi-cellular structures and functional tissue.

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