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Kostovska I, Tosheska-Trajkovska K, Topuzovska S, et al. Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria. J Med Biochem. 2020;39(1):83-90doi: 10.2478/jomb-2019-0026.
Kostovska, I., Tosheska-Trajkovska, K., Topuzovska, S., Cekovska, S., Spasovski, G., Kostovski, O., & Labudovic, D. (2020). Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria. Journal of medical biochemistry, 39(1), 83-90. https://doi.org/10.2478/jomb-2019-0026
Kostovska, Irena, et al. "Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria." Journal of medical biochemistry vol. 39,1 (2020): 83-90. doi: https://doi.org/10.2478/jomb-2019-0026
Kostovska I, Tosheska-Trajkovska K, Topuzovska S, Cekovska S, Spasovski G, Kostovski O, Labudovic D. Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria. J Med Biochem. 2020 Jan 10;39(1):83-90. doi: 10.2478/jomb-2019-0026. PMID: 32549781; PMCID: PMC7282240.
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J Med Biochem. 2020 Jan 10;39(1):83-90. doi: 10.2478/jomb-2019-0026.

Urinary nephrin is earlier, more sensitive and specific marker of diabetic nephropathy than microalbuminuria.

Journal of medical biochemistry

Irena Kostovska, Katerina Tosheska-Trajkovska, Sonja Topuzovska, Svetlana Cekovska, Goce Spasovski, Ognen Kostovski, Danica Labudovic

Affiliations

  1. Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. Macedonia.
  2. Medical Faculty, University Department of Nephrology, Skopje, R. Macedonia.
  3. Medical Faculty, University Clinic of Abdominal Surgery, Skopje, R. Macedonia.

PMID: 32549781 PMCID: PMC7282240 DOI: 10.2478/jomb-2019-0026

Abstract

BACKGROUND: Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. Progressive damage and decline in the number of podocytes often occur in the early stages of DN. Thus, nephrin as a podocyte-specific protein may be regarded as a potential biomarker of early detection of DN. The aim of this study is to determine whether urinary nephrin is an earlier marker in DN than microalbuminuria and to test the significance of urinary nephrin as a marker for early detection of DN.

METHODS: Our cross-sectional study included 90 patients with type 2 diabetes mellitus (T2DM), 30 patients with diagnosed DN and 60 patients without diagnosed DN. As a control group, we used 30 healthy subjects. All patients with T2DM were classified into three subgroups according to urinary microalbumin/creatinine ratio (UMCR): normoalbuminuric, microalbuminuric and macroalbuminuric patients. Nephrin in urine was measured by immunoenzyme assay, microalbumin with turbidimetric and creatinine with the photometric method. In blood sera, we measured a few standard biochemical parameters.

RESULTS: Nephrinuria was found to be present in 100% of patients with T2DM and macroalbuminuria, in 88% with microalbuminuria, as well as 82% of patients with T2DM and normoalbuminuria. A concentration of urinary nephrin was significantly increased in all groups of subjects with T2DM compared to the control group (p<0.05). Nephrinuria correlated statistically negative with eGFR (r=-0.54). ROC analysis showed that nephrin has a total predicted probability of 96% in patients with DN.

CONCLUSIONS: Urinary nephrin is earlier, more specific and sensitive marker than microalbumin in early detection of DN.

2020 Irena Kostovska, Katerina Tosheska-Trajkovska, Sonja Topuzovska, Svetlana Cekovska, Goce Spasovski, Ognen Kostovski, Danica Labudovic, published by CEON/CEES.

Keywords: diabetic nephropathy; microalbumin; nephrin

Conflict of interest statement

Conflict of Interest: The authors stated that they have no conflicts of interest regarding the publication of this article.

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