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Elitas M, Sengul E. Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics. Micromachines (Basel). 2020;11(6)doi: 10.3390/mi11060576.
Elitas, M., & Sengul, E. (2020). Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics. Micromachines, 11(6), . https://doi.org/10.3390/mi11060576
Elitas, Meltem, and Sengul, Esra. "Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics." Micromachines vol. 11,6 (2020). doi: https://doi.org/10.3390/mi11060576
Elitas M, Sengul E. Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics. Micromachines (Basel). 2020 Jun 08;11(6). doi: 10.3390/mi11060576. PMID: 32521676; PMCID: PMC7345647.
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Micromachines (Basel). 2020 Jun 08;11(6). doi: 10.3390/mi11060576.

Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics.

Micromachines

Meltem Elitas, Esra Sengul

Affiliations

  1. Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, Turkey.
  2. Sabanci University Nanotechnology Research and Application Center, Istanbul 34956, Turkey.

PMID: 32521676 PMCID: PMC7345647 DOI: 10.3390/mi11060576

Abstract

A variety of force fields have thus far been demonstrated to investigate electromechanical properties of cells in a microfluidic platform which, however, are mostly based on fluid shear stress and may potentially cause irreversible cell damage. This work presents dielectric movement and deformation measurements of U937 monocytes and U937-differentiated macrophages in a low conductive medium inside a 3D carbon electrode array. Here, monocytes exhibited a crossover frequency around 150 kHz and presented maximum deformation index at 400 kHz and minimum deformation index at 1 MHz frequencies at 20 V

Keywords: deformation; dielectrophoresis; heterogeneity; macrophage; mobility; monocyte

Conflict of interest statement

The authors declare no conflict of interest.

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