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Garcia IS, Teixeira SA, Costa KA, et al. l-Arginine supplementation of gilts during early gestation modulates energy sensitive pathways in pig conceptuses. Mol Reprod Dev. 2020;doi: 10.1002/mrd.23397.
Garcia, I. S., Teixeira, S. A., Costa, K. A., Marques, D. B. D., Rodrigues, G. A., Costa, T. C., Guimarães, J. D., Otto, P. I., Saraiva, A., Ibelli, A. M. G., Cantão, M. E., de Oliveira, H. C., Ledur, M. C., Peixoto, J. O., & Guimarães, S. E. F. (2020). l-Arginine supplementation of gilts during early gestation modulates energy sensitive pathways in pig conceptuses. Molecular reproduction and development, . https://doi.org/10.1002/mrd.23397
Garcia, Ingrid S, et al. "l-Arginine supplementation of gilts during early gestation modulates energy sensitive pathways in pig conceptuses." Molecular reproduction and development vol. (2020). doi: https://doi.org/10.1002/mrd.23397
Garcia IS, Teixeira SA, Costa KA, Marques DBD, Rodrigues GA, Costa TC, Guimarães JD, Otto PI, Saraiva A, Ibelli AMG, Cantão ME, de Oliveira HC, Ledur MC, Peixoto JO, Guimarães SEF. l-Arginine supplementation of gilts during early gestation modulates energy sensitive pathways in pig conceptuses. Mol Reprod Dev. 2020 Jun 27; doi: 10.1002/mrd.23397. Epub 2020 Jun 27. PMID: 32592179.
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Mol Reprod Dev. 2020 Jun 27; doi: 10.1002/mrd.23397. Epub 2020 Jun 27.

l-Arginine supplementation of gilts during early gestation modulates energy sensitive pathways in pig conceptuses.

Molecular reproduction and development

Ingrid S Garcia, Susana A Teixeira, Karine A Costa, Daniele B D Marques, Gustavo de A Rodrigues, Thaís C Costa, José D Guimarães, Pamela I Otto, Alysson Saraiva, Adriana M G Ibelli, Maurício E Cantão, Haniel C de Oliveira, Mônica C Ledur, Jane de O Peixoto, Simone E F Guimarães

Affiliations

  1. Department of Animal Science, Universidade Federal de Viçosa, Viçosa, Brazil.
  2. Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Brazil.
  3. Animal Genetics Laboratory, Embrapa Swine and Poultry Nacional Research Center, Concordia, Brazil.

PMID: 32592179 DOI: 10.1002/mrd.23397

Abstract

Dietary l-arginine (ARG) supplementation has been studied as a nutritional strategy to improve reproductive performance of pregnant sows, since arginine is a conditionally essential amino acid. However, reports addressing the molecular mechanisms that mediate supplementation effects on embryos and fetuses development are still scarce. Therefore, we aimed to evaluate the effects of 1.0% ARG supplementation of commercial pregnant gilts on genes and proteins from energy metabolism and antioxidant defense pathways in embryos and fetuses. We also analyzed the global transcriptome profile of 25- and 35-day-old conceptuses. At Day 25, we observed a lower abundance of phospho-AMP-activated protein kinase (phospho-AMPK) protein and downregulation of oxidative phosphorylation system genes in ARG embryos. On the other hand, ARG fetuses showed greater expression of MLST8 and lower expression of MTOR genes, in addition to lower abundance of phospho-AMPK and phospho-mammalian target of rapamycin (phospho-mTOR) proteins. Transcriptome analysis at Day 35 did not present differentially expressed genes. For the antioxidant defense pathway, no differences were found between CON and ARG conceptuses, only trends. In general, supplementation of gilts with 1.0% ARG during early gestation affects energy sensitive pathways in 25- and 35-day conceptuses; however, no effects of supplementation were found on the antioxidative defense pathway in 25-day embryos.

© 2020 Wiley Periodicals LLC.

Keywords: RNA-seq; amino acid supplementation; antioxidants; fetal programming; mTOR pathway; nutrigenomics

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