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Stem Cell Res. 2020 Jun 24;47:101891. doi: 10.1016/j.scr.2020.101891. Epub 2020 Jun 24.

Establishment of a human induced pluripotent stem cell (iPSC) line (JUCTCi010-A) from a healthy Jordanian female skin dermal fibroblasts.

Stem cell research

Nidaa A Ababneh, Ban Al-Kurdi, Dema Ali, Duaa Abuarqoub, Raghda Barham, Bareqa Salah, Abdalla Awidi

Affiliations

  1. Cell Therapy Center, the University of Jordan, Amman, Jordan. Electronic address: [email protected].
  2. Cell Therapy Center, the University of Jordan, Amman, Jordan.
  3. Cell Therapy Center, the University of Jordan, Amman, Jordan; Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
  4. General Surgery Department/Plastic & Reconstructive, Jordan University Hospital, The University of Jordan, Amman, Jordan.
  5. Cell Therapy Center, the University of Jordan, Amman, Jordan; Hemostasis and Thrombosis Laboratory, School of Medicine, the University of Jordan, Amman, Jordan; Department of Hematology and Oncology, Jordan University Hospital, Amman, Jordan.

PMID: 32622344 DOI: 10.1016/j.scr.2020.101891

Abstract

Human integration-free induced pluripotent pluripotent stem cells (hiPSCs) were generated from skin fibroblasts obtained from a 27-year-old healthy Jordanian female. The resulting iPSCs expressed the most common pluripotency stem cell markers, they retained the normal karyotype similar to the original fibroblasts and showed the potential to differentiate into three germ layers in vitro. This iPSC line could serve as a wild-type control that can be used in hereditary disease modeling studies and in optimization of different differentiation protocols.

Copyright © 2020. Published by Elsevier B.V.

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