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Kazan A, Heymuth M, Karabulut D, et al. Formulation of organic and inorganic hydrogel matrices for immobilization of β-glucosidase in microfluidic platform. Eng Life Sci. 2017;17(7):714-722doi: 10.1002/elsc.201600218.
Kazan, A., Heymuth, M., Karabulut, D., Akay, S., Yildiz-Ozturk, E., Onbas, R., Muderrisoglu, C., Sargin, S., Heils, R., Smirnova, I., & Yesil-Celiktas, O. (2017). Formulation of organic and inorganic hydrogel matrices for immobilization of β-glucosidase in microfluidic platform. Engineering in life sciences, 17(7), 714-722. https://doi.org/10.1002/elsc.201600218
Kazan, Aslihan, et al. "Formulation of organic and inorganic hydrogel matrices for immobilization of β-glucosidase in microfluidic platform." Engineering in life sciences vol. 17,7 (2017): 714-722. doi: https://doi.org/10.1002/elsc.201600218
Kazan A, Heymuth M, Karabulut D, Akay S, Yildiz-Ozturk E, Onbas R, Muderrisoglu C, Sargin S, Heils R, Smirnova I, Yesil-Celiktas O. Formulation of organic and inorganic hydrogel matrices for immobilization of β-glucosidase in microfluidic platform. Eng Life Sci. 2017 Feb 27;17(7):714-722. doi: 10.1002/elsc.201600218. eCollection 2017 Jul. PMID: 32624816; PMCID: PMC6999321.
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Eng Life Sci. 2017 Feb 27;17(7):714-722. doi: 10.1002/elsc.201600218. eCollection 2017 Jul.

Formulation of organic and inorganic hydrogel matrices for immobilization of β-glucosidase in microfluidic platform.

Engineering in life sciences

Aslihan Kazan, Marcel Heymuth, Dilan Karabulut, Seref Akay, Ece Yildiz-Ozturk, Rabia Onbas, Cahit Muderrisoglu, Sayit Sargin, Rene Heils, Irina Smirnova, Ozlem Yesil-Celiktas

Affiliations

  1. Department of Bioengineering Faculty of Engineering Ege University Izmir Turkey.
  2. Institute of Thermal Separation Processes Hamburg University of Technology Hamburg Germany.

PMID: 32624816 PMCID: PMC6999321 DOI: 10.1002/elsc.201600218

Abstract

The aim of this study was to formulate silica and alginate hydrogels for immobilization of β-glucosidase. For this purpose, enzyme kinetics in hydrogels were determined, activity of immobilized enzymes was compared with that of free enzyme, and structures of silica and alginate hydrogels were characterized in terms of surface area and pore size. The addition of polyethylene oxide improved the mechanical strength of the silica gels and 68% of the initial activity of the enzyme was preserved after immobilizing into tetraethyl orthosilicate-polyethylene oxide matrix where the relative activity in alginate beads was 87%. The immobilized β-glucosidase was loaded into glass-silicon-glass microreactors and catalysis of 4-nitrophenyl β-d-glucopyranoside was carried out at various retention times (5, 10, and 15 min) to compare the performance of silica and alginate hydrogels as immobilization matrices. The results indicated that alginate hydrogels exhibited slightly better properties than silica, which can be utilized for biocatalysis in microfluidic platforms.

© 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: Enzyme; Hydrogels; Immobilization; Microreactor

References

  1. Biotechnol Bioeng. 1996 Mar 5;49(5):527-34 - PubMed
  2. Carbohydr Polym. 2014 Jun 15;106:94-100 - PubMed
  3. Prog Polym Sci. 2012 Jan;37(1):106-126 - PubMed
  4. Macromol Biosci. 2006 Aug 7;6(8):623-33 - PubMed
  5. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9287-92 - PubMed
  6. Enzyme Microb Technol. 2013 Mar 5;52(3):141-50 - PubMed
  7. Anal Biochem. 2008 Feb 15;373(2):272-80 - PubMed
  8. Biochem Eng J. 2001 Jul;8(1):51-59 - PubMed
  9. Anal Chem. 2005 Apr 1;77(7):1950-6 - PubMed
  10. Anal Biochem. 2011 Jul 15;414(2):226-31 - PubMed
  11. Anal Biochem. 2012 Aug 15;427(2):139-43 - PubMed

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