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Prakash K, Larsson SB, Rydell GE, et al. Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction. Hepatol Commun. 2020;4(7):973-982doi: 10.1002/hep4.1507.
Prakash, K., Larsson, S. B., Rydell, G. E., Andersson, M. E., Ringlander, J., Norkrans, G., Norder, H., & Lindh, M. (2020). Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction. Hepatology communications, 4(7), 973-982. https://doi.org/10.1002/hep4.1507
Prakash, Kasthuri, et al. "Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction." Hepatology communications vol. 4,7 (2020): 973-982. doi: https://doi.org/10.1002/hep4.1507
Prakash K, Larsson SB, Rydell GE, Andersson ME, Ringlander J, Norkrans G, Norder H, Lindh M. Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction. Hepatol Commun. 2020 Mar 29;4(7):973-982. doi: 10.1002/hep4.1507. eCollection 2020 Jul. PMID: 32626830; PMCID: PMC7327224.
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Hepatol Commun. 2020 Mar 29;4(7):973-982. doi: 10.1002/hep4.1507. eCollection 2020 Jul.

Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain Reaction.

Hepatology communications

Kasthuri Prakash, Simon B Larsson, Gustaf E Rydell, Maria E Andersson, Johan Ringlander, Gunnar Norkrans, Heléne Norder, Magnus Lindh

Affiliations

  1. Department of Infectious Diseases Sahlgrenska Academy University of Gothenburg Gothenburg Sweden.

PMID: 32626830 PMCID: PMC7327224 DOI: 10.1002/hep4.1507

Abstract

Replication of hepatitis B virus (HBV) originates from covalently closed circular DNA (cccDNA) and involves reverse transcription of pregenomic RNA (pgRNA), which is also called core RNA and encodes the capsid protein. The RNA coding for hepatitis B surface antigen (HBsAg) in the envelope of viral or subviral particles is produced from cccDNA or from HBV DNA integrated into the host genome. Because only cccDNA can generate the core and the 3' redundancy regions of HBV RNA, we aimed to clarify to what extent such HBV integrations are expressed by quantifying the different HBV RNA species in liver tissue. Digital droplet polymerase chain reaction (ddPCR) was employed to quantify six HBV RNA targets in 76 liver biopsies from patients with chronic infection, comprising 14 who were hepatitis B e antigen (HBeAg) positive and 62 who were HBeAg negative. In patients who were HBeAg negative, HBV RNA from the S RNA region was >1.6 log

© 2020 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

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