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Beck L, Su J, Comerma-Steffensen S, et al. Endothelial Dysfunction and Passive Changes in the Aorta and Coronary Arteries of Diabetic db/db Mice. Front Physiol. 2020;11:667doi: 10.3389/fphys.2020.00667.
Beck, L., Su, J., Comerma-Steffensen, S., Pinilla, E., Carlsson, R., Hernanz, R., Sheykhzade, M., Danielsen, C. C., & Simonsen, U. (2020). Endothelial Dysfunction and Passive Changes in the Aorta and Coronary Arteries of Diabetic db/db Mice. Frontiers in physiology, 11667. https://doi.org/10.3389/fphys.2020.00667
Beck, Lilliana, et al. "Endothelial Dysfunction and Passive Changes in the Aorta and Coronary Arteries of Diabetic db/db Mice." Frontiers in physiology vol. 11 (2020): 667. doi: https://doi.org/10.3389/fphys.2020.00667
Beck L, Su J, Comerma-Steffensen S, Pinilla E, Carlsson R, Hernanz R, Sheykhzade M, Danielsen CC, Simonsen U. Endothelial Dysfunction and Passive Changes in the Aorta and Coronary Arteries of Diabetic db/db Mice. Front Physiol. 2020 Jun 23;11:667. doi: 10.3389/fphys.2020.00667. eCollection 2020. PMID: 32655412; PMCID: PMC7324802.
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Front Physiol. 2020 Jun 23;11:667. doi: 10.3389/fphys.2020.00667. eCollection 2020.

Endothelial Dysfunction and Passive Changes in the Aorta and Coronary Arteries of Diabetic db/db Mice.

Frontiers in physiology

Lilliana Beck, Junjing Su, Simon Comerma-Steffensen, Estéfano Pinilla, Rune Carlsson, Raquel Hernanz, Majid Sheykhzade, Carl Christian Danielsen, Ulf Simonsen

Affiliations

  1. Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Faculty of Health Aarhus University, Aarhus, Denmark.
  2. Department of Biomedical Sciences/Animal Physiology, Veterinary Faculty, Central University of Venezuela, Maracay, Venezuela.
  3. Departamento de Ciencias Básicas de la Salud, Universidad Rey Juan Carlos, Alcorcón, Spain.
  4. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

PMID: 32655412 PMCID: PMC7324802 DOI: 10.3389/fphys.2020.00667

Abstract

Endothelial cell dysfunction and vessel stiffening are associated with a worsened prognosis in diabetic patients with cardiovascular diseases. The present study hypothesized that sex impacts endothelial dysfunction and structural changes in arteries from diabetic mice. In diabetic (db/db) and normoglycaemic (db/db+) mice, the mechanical properties were investigated in pressurized isolated left anterior descending coronary arteries and aorta segments that were subjected to tensile testing. Functional studies were performed on wire-mounted vascular segments. The male and female db/db mice were hyperglycaemic and had markedly increased body weight. In isolated aorta segments without the contribution of smooth muscle cells, load to rupture, viscoelasticity, and collagen content were decreased suggesting larger distensibility of the arterial wall in both male and female db/db mice. In male db/db aorta segments with smooth muscle cell contribution, lumen diameter was smaller and the passive stretch-tension curve was leftward-shifted, while they were unaltered in female db/db aorta segments versus control db/db+ mice. In contrast to female db/db mice, coronary arteries from male db/db mice had altered stress-strain relationships and increased distensibility. Transthoracic echocardiography revealed a dilated left ventricle with unaltered cardiac output, while aortic flow velocity was decreased in male db/db mice. Impairment of acetylcholine relaxation was aggravated in aorta from female db/db compared to control and male db/db mice, while impairment of sodium nitroprusside relaxations was only observed in aorta from male db/db mice. The remodeling in the coronary arteries and aorta suggests an adaptation of the arterial wall to the reduced flow velocity with sex-specific differences in the passive properties of aorta and coronary arteries. The findings of less distensible arteries and more pronounced endothelial dysfunction in female compared to male diabetic mice may have implications for the observed higher incidence of macrovascular complications in diabetic women.

Copyright © 2020 Beck, Su, Comerma-Steffensen, Pinilla, Carlsson, Hernanz, Sheykhzade, Danielsen and Simonsen.

Keywords: acetylcholine; aorta; coronary arteries; endothelium; stiffness; viscoelasticity

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