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Utomo B, Putranto ED, Fadholly A. Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction. Vet World. 2020;13(5):879-883doi: 10.14202/vetworld.2020.879-883.
Utomo, B., Putranto, E. D., & Fadholly, A. (2020). Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction. Veterinary world, 13(5), 879-883. https://doi.org/10.14202/vetworld.2020.879-883
Utomo, Budi, et al. "Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction." Veterinary world vol. 13,5 (2020): 879-883. doi: https://doi.org/10.14202/vetworld.2020.879-883
Utomo B, Putranto ED, Fadholly A. Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction. Vet World. 2020 May;13(5):879-883. doi: 10.14202/vetworld.2020.879-883. Epub 2020 May 11. PMID: 32636582; PMCID: PMC7311873.
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Vet World. 2020 May;13(5):879-883. doi: 10.14202/vetworld.2020.879-883. Epub 2020 May 11.

Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction.

Veterinary world

Budi Utomo, Emmanuel Djoko Putranto, Amaq Fadholly

Affiliations

  1. Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
  2. Department of Veterinary Clinical, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.

PMID: 32636582 PMCID: PMC7311873 DOI: 10.14202/vetworld.2020.879-883

Abstract

BACKGROUND AND AIM: The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism.

MATERIALS AND METHODS: Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism.

RESULTS: Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188.

CONCLUSION: Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes.

Copyright: © Utomo, et al.

Keywords: Madrasin; follicle-stimulating hormone; follicle-stimulating hormone receptors; hypofunction

References

  1. PLoS One. 2013 Oct 22;8(10):e78138 - PubMed
  2. Anim Reprod Sci. 2010 Jun;119(3-4):172-7 - PubMed
  3. Anim Reprod Sci. 2018 Jan;188:45-50 - PubMed
  4. J Assist Reprod Genet. 2015 Nov;32(11):1659-68 - PubMed
  5. Genetika. 2015 Nov;51(11):1264-72 - PubMed
  6. Front Endocrinol (Lausanne). 2019 May 14;10:305 - PubMed
  7. J Anim Sci Technol. 2018 Dec 12;60:31 - PubMed
  8. Nat Commun. 2015 Oct 20;6:8645 - PubMed
  9. Front Endocrinol (Lausanne). 2019 Feb 12;10:23 - PubMed
  10. Mol Genet Metab. 2011 Aug;103(4):388-93 - PubMed
  11. J Vet Diagn Invest. 2015 Sep;27(5):596-9 - PubMed
  12. Front Vet Sci. 2016 Oct 18;3:94 - PubMed
  13. J Vet Med Sci. 2016 Feb;78(2):281-6 - PubMed
  14. Reprod Sci. 2013 Mar;20(3):211-33 - PubMed
  15. Clin Endocrinol (Oxf). 2015 Apr;82(4):577-83 - PubMed
  16. Endocrinology. 2017 Jul 1;158(7):2043-2051 - PubMed

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