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Almonacid Suarez AM, van der Ham I, Brinker MGL, et al. Topography-driven alterations in endothelial cell phenotype and contact guidance. Heliyon. 2020;6(6):e04329doi: 10.1016/j.heliyon.2020.e04329.
Almonacid Suarez, A. M., van der Ham, I., Brinker, M. G. L., van Rijn, P., & Harmsen, M. C. (2020). Topography-driven alterations in endothelial cell phenotype and contact guidance. Heliyon, 6(6), e04329. https://doi.org/10.1016/j.heliyon.2020.e04329
Almonacid Suarez, Ana Maria, et al. "Topography-driven alterations in endothelial cell phenotype and contact guidance." Heliyon vol. 6,6 (2020): e04329. doi: https://doi.org/10.1016/j.heliyon.2020.e04329
Almonacid Suarez AM, van der Ham I, Brinker MGL, van Rijn P, Harmsen MC. Topography-driven alterations in endothelial cell phenotype and contact guidance. Heliyon. 2020 Jun 29;6(6):e04329. doi: 10.1016/j.heliyon.2020.e04329. eCollection 2020 Jun. PMID: 32637708; PMCID: PMC7330714.
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Heliyon. 2020 Jun 29;6(6):e04329. doi: 10.1016/j.heliyon.2020.e04329. eCollection 2020 Jun.

Topography-driven alterations in endothelial cell phenotype and contact guidance.

Heliyon

Ana Maria Almonacid Suarez, Iris van der Ham, Marja G L Brinker, Patrick van Rijn, Martin C Harmsen

Affiliations

  1. University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Hanzeplein 1 (EA11), 9713 GZ, Groningen, the Netherlands.
  2. University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering-FB40, W.J. Kolff Institute for Biomedical Engineering and Materials Science-FB41, A. Deusinglaan 1, 9713 AV, Groningen, the Netherlands.

PMID: 32637708 PMCID: PMC7330714 DOI: 10.1016/j.heliyon.2020.e04329

Abstract

Understanding how endothelial cell phenotype is affected by topography could improve the design of new tools for tissue engineering as many tissue engineering approaches make use of topography-mediated cell stimulation. Therefore, we cultured human pulmonary microvascular endothelial cells (ECs) on a directional topographical gradient to screen the EC vascular-like network formation and alignment response to nano to microsized topographies. The cell response was evaluated by microscopy. We found that ECs formed unstable vascular-like networks that aggregated in the smaller topographies and flat parts whereas ECs themselves aligned on the larger topographies. Subsequently, we designed a mixed topography where we could explore the network formation and proliferative properties of these ECs by live imaging for three days. Vascular-like network formation continued to be unstable on the topography and were only produced on the flat areas and a fibronectin coating did not improve the network stability. However, an instructive adipose tissue-derived stromal cell (ASC) coating provided the correct environment to sustain the vascular-like networks, which were still affected by the topography underneath. It was concluded that large microsized topographies inhibit vascular endothelial network formation but not proliferation and flat and nano/microsized topographies allow formation of early networks that can be stabilized by using an ASC instructive layer.

© 2020 The Author(s).

Keywords: Bioengineering; Biomedical engineering; Biophysics; Cell biology; Contact guidance; Directional topography; Endothelial cells; Regenerative medicine; Vascular-like networks; Vascularization

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