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Menezes BS, Solidade LS, Conceição AA, et al. Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties. AMB Express. 2020;10(1):117doi: 10.1186/s13568-020-01054-y.
Menezes, B. S., Solidade, L. S., Conceição, A. A., Santos Junior, M. N., Leal, P. L., de Brito, E. S., Canuto, K. M., Mendonça, S., de Siqueira, F. G., & Marques, L. M. (2020). Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties. AMB Express, 10(1), 117. https://doi.org/10.1186/s13568-020-01054-y
Menezes, Bruna S, et al. "Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties." AMB Express vol. 10,1 (2020): 117. doi: https://doi.org/10.1186/s13568-020-01054-y
Menezes BS, Solidade LS, Conceição AA, Santos Junior MN, Leal PL, de Brito ES, Canuto KM, Mendonça S, de Siqueira FG, Marques LM. Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties. AMB Express. 2020 Jul 01;10(1):117. doi: 10.1186/s13568-020-01054-y. PMID: 32613282; PMCID: PMC7329961.
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AMB Express. 2020 Jul 01;10(1):117. doi: 10.1186/s13568-020-01054-y.

Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties.

AMB Express

Bruna S Menezes, Lucas S Solidade, Aparecido A Conceição, Manoel N Santos Junior, Patrícia L Leal, Edy S de Brito, Kirley M Canuto, Simone Mendonça, Félix G de Siqueira, Lucas M Marques

Affiliations

  1. Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia. Vitória da Conquista, Bahia, Brazil.
  2. Embrapa Agroindústria Tropical, Fortaleza, Ceará, Brazil.
  3. Embrapa Agroenergia, Distrito Federal, Brasília, Brazil.
  4. Embrapa Agroenergia, Distrito Federal, Brasília, Brazil. [email protected].
  5. Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia. Vitória da Conquista, Bahia, Brazil. [email protected].

PMID: 32613282 PMCID: PMC7329961 DOI: 10.1186/s13568-020-01054-y

Abstract

The fungal kingdom has been widely studied as a source of bioactive compounds of interest to the pharmaceutical and food industry. This paper studies the production of natural red pigments by Fusarium solani BRM054066 in the submerged fermentation system, using Doehlert experimental design to determine optimal cultivation conditions. The chemical composition of the red pigment was determined by Nuclear Magnetic Resonance spectroscopy (NMR) and Ultra-Performance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MS). Antioxidant activity was assessed by the ability to sequester of free radical DPPH. In the analysis of anti-inflammatory activity, murine peritoneal macrophages activated by LPS were used, and the gene expression of TNF-α, IL-1β, IL-6, IL-10 and IL-17 was determined using qPCR. As a result, it was found that agitation at 200 rpm and glucose concentration ≥ 20 g/L promote the best results in the production of red pigment. The chemical compounds identified were two naphthoquinones, fusarubin and dihydrofusarubin, and an anthraquinone, a bostrycoidin, being fusarubin the majority compound. The red pigment showed antioxidant activity by scavenge 50% of the DPPH radical, in a concentration of 24 µg/mL. The pigment also showed an effective anti-inflammatory capacity by reducing the overexpression of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 and promoting the production of anti-inflammatory IL-10 and IL-17, in murine macrophages activated by LPS (p < 0.05). According to the results, the fungus F. solani BRM054066, under optimized conditions of cultivation, proved to be a promising source of biologically active natural pigments with wide industrial applicability.

Keywords: Bioactive compounds; Doehlert design; Natural red pigment; Submerged fermentation

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