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Holzer B, Manoli K, Ditaranto N, et al. Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes. Adv Biosyst. 2017;1(11):e1700055doi: 10.1002/adbi.201700055.
Holzer, B., Manoli, K., Ditaranto, N., Macchia, E., Tiwari, A., Di Franco, C., Scamarcio, G., Palazzo, G., & Torsi, L. (2017). Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes. Advanced biosystems, 1(11), e1700055. https://doi.org/10.1002/adbi.201700055
Holzer, Brigitte, et al. "Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes." Advanced biosystems vol. 1,11 (2017): e1700055. doi: https://doi.org/10.1002/adbi.201700055
Holzer B, Manoli K, Ditaranto N, Macchia E, Tiwari A, Di Franco C, Scamarcio G, Palazzo G, Torsi L. Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes. Adv Biosyst. 2017 Nov;1(11):e1700055. doi: 10.1002/adbi.201700055. Epub 2017 Jun 05. PMID: 32646170.
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Adv Biosyst. 2017 Nov;1(11):e1700055. doi: 10.1002/adbi.201700055. Epub 2017 Jun 05.

Characterization of Covalently Bound Anti-Human Immunoglobulins on Self-Assembled Monolayer Modified Gold Electrodes.

Advanced biosystems

Brigitte Holzer, Kyriaki Manoli, Nicoletta Ditaranto, Eleonora Macchia, Amber Tiwari, Cinzia Di Franco, Gaetano Scamarcio, Gerardo Palazzo, Luisa Torsi

Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Bari - "Aldo Moro", Via Orabona 4, 70126, Bari, Italy.
  2. CSGI (Center for Colloid and Surface Science) - Bari, Via Orabona 4, 70126, Bari, Italy.
  3. CNR - Istituto di Fotonica e Nanotecnologie, Sede di Bari, Via Orabona 4, 70126, Bari, Italy.
  4. Dipartimento di Fisica "M. Merlin" - Università degli Studi di Bari - "Aldo Moro", Via Orabona 4, 70126, Bari, Italy.

PMID: 32646170 DOI: 10.1002/adbi.201700055

Abstract

Bioconjugated gold surfaces constitute interesting platforms for biosensing applications. The immobilization of antibodies such as anti-immunoglobulin G and M (anti-IgG and anti-IgM) on gold electrodes via self-assembled monolayers (SAMs) is here studied as a model system for further immunoassays development. The biolayer is characterized by means of X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), a dedicated thin-film transistor (TFT)-based platform and electrochemical surface plasmon resonance (EC-SPR). XPS analysis confirms the presence of all the chemical species involved in the fabrication process as well as the covalent attachment of the antibodies with high reproducibility. Visualization of the biolayer topography by AFM shows nanostructures with a thickness consistent with the actual size of the protein, which is also verified by SPR measurements. EC-SPR allows taking advantage of complementary electrochemical and optical signals during the functionalization steps. Moreover, the functionalization of gold leads to a change in the work function, which is demonstrated in an electrolyte gated thin-film transistor configuration. Such configuration enables also to evaluate the electrostatic changes occurring on the gate that are connected with the threshold voltage shifts. The data support that functional biomodified gold surfaces can be reproducibly prepared, which is a prerequisite for further biosensor development.

© 2017 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: biosensors; immunoglobulin; self-assembled monolayers; surface analysis; thin-film transistors

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