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Zhang S, Li G, Man J, et al. Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle. Micromachines (Basel). 2020;11(7)doi: 10.3390/mi11070681.
Zhang, S., Li, G., Man, J., Zhang, S., Li, J., Li, J., & Li, D. (2020). Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle. Micromachines, 11(7), . https://doi.org/10.3390/mi11070681
Zhang, Shanguo, et al. "Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle." Micromachines vol. 11,7 (2020). doi: https://doi.org/10.3390/mi11070681
Zhang S, Li G, Man J, Zhang S, Li J, Li J, Li D. Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle. Micromachines (Basel). 2020 Jul 14;11(7). doi: 10.3390/mi11070681. PMID: 32674334; PMCID: PMC7408603.
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Micromachines (Basel). 2020 Jul 14;11(7). doi: 10.3390/mi11070681.

Fabrication of Microspheres from High-Viscosity Bioink Using a Novel Microfluidic-Based 3D Bioprinting Nozzle.

Micromachines

Shanguo Zhang, Guiling Li, Jia Man, Song Zhang, Jianyong Li, Jianfeng Li, Donghai Li

Affiliations

  1. Key Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, China.
  2. Key National Demonstration Center for Experimental Mechanical Engineering Education, Shandong University, Jinan 250061, China.
  3. School of Medicine, Tsinghua University, Beijing 100084, China.
  4. Advanced Medical Research Institute, Shandong University, Jinan 250012, China.

PMID: 32674334 PMCID: PMC7408603 DOI: 10.3390/mi11070681

Abstract

Three-dimensional (3D) bioprinting is a novel technology utilizing biocompatible materials, cells, drugs, etc. as basic microcomponents to form 3D artificial structures and is believed as a promising method for regenerative medicine. Droplet-based bioprinting can precisely generate microspheres and manipulate them into organized structures with high fidelity. Biocompatible hydrogels are usually used as bioinks in 3D bioprinting, however, the viscosity of the bioink could be increased due to the additives such as cells, drugs, nutrient factors and other functional polymers in some particular applications, making it difficult to form monodispersed microspheres from high-viscosity bioink at the orifice of the nozzle. In this work, we reported a novel microfluidic-based printing nozzle to prepare monodispersed microspheres from high-viscosity bioink using the phase-inversion method. Different flowing conditions can be achieved by changing the flow rates of the fluids to form monodispersed solid and hollow microspheres using the same nozzle. The diameter of the microspheres can be tuned by changing the flow rate ratio and the size distribution of the microspheres is narrow. The prepared calcium alginate microspheres could also act as micro-carriers in drug delivery.

Keywords: droplet-based bioprinting; microcomponent; microfluidic system; phase-inversion method

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