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Mantecón-Oria M, Diban N, Berciano MT, et al. Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood-Brain Barrier Models. Membranes (Basel). 2020;10(8)doi: 10.3390/membranes10080161.
Mantecón-Oria, M., Diban, N., Berciano, M. T., Rivero, M. J., David, O., Lafarga, M., Tapia, O., & Urtiaga, A. (2020). Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood-Brain Barrier Models. Membranes, 10(8), . https://doi.org/10.3390/membranes10080161
Mantecón-Oria, Marián, et al. "Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood-Brain Barrier Models." Membranes vol. 10,8 (2020). doi: https://doi.org/10.3390/membranes10080161
Mantecón-Oria M, Diban N, Berciano MT, Rivero MJ, David O, Lafarga M, Tapia O, Urtiaga A. Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood-Brain Barrier Models. Membranes (Basel). 2020 Jul 22;10(8). doi: 10.3390/membranes10080161. PMID: 32708027; PMCID: PMC7464335.
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Membranes (Basel). 2020 Jul 22;10(8). doi: 10.3390/membranes10080161.

Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood-Brain Barrier Models.

Membranes

Marián Mantecón-Oria, Nazely Diban, Maria T Berciano, Maria J Rivero, Oana David, Miguel Lafarga, Olga Tapia, Ane Urtiaga

Affiliations

  1. Department of Chemical and Biomolecular Engineering, ETSIIyT, University of Cantabria, Avda. Los Castros s/n, 39005 Santander, Spain.
  2. Instituto de Investigación Marqués de Valdecilla (IDIVAL), Cardenal H. Oria s/n, 39011 Santander, Spain.
  3. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 528031 Madrid, Spain.
  4. Department of Molecular Biology, University of Cantabria, Cardenal H. Oria s/n, 39011 Santander, Spain.
  5. TECNALIA, Basque Research and Technology Alliance (BRTA), Mikeletegi Pasealekua 2, 20009 San Sebastián, Spain.
  6. Department of Anatomy and Cell Biology, University of Cantabria, Cardenal H. Oria s/n, 39011 Santander, Spain.
  7. Universidad Europea del Atlántico, Parque Científico y Tecnológico de Cantabria, Isabel Torres 21, 39011 Santander, Spain.

PMID: 32708027 PMCID: PMC7464335 DOI: 10.3390/membranes10080161

Abstract

There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood-brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems.

Keywords: 3D cell cultures; graphene; in vitro blood brain barrier (BBB) model; mixed-matrix hollow fibers; poly(ε-caprolactone)

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