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Kroeck KG, Qiu W, Catalano C, et al. Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. J Vis Exp. 2020;(161)doi: 10.3791/61298.
Kroeck, K. G., Qiu, W., Catalano, C., Trinh, T. K. H., & Guo, Y. (2020). Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. Journal of visualized experiments : JoVE, (161), . https://doi.org/10.3791/61298
Kroeck, Kyle G, et al. "Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis." Journal of visualized experiments : JoVE vol. ,161 (2020). doi: https://doi.org/10.3791/61298
Kroeck KG, Qiu W, Catalano C, Trinh TKH, Guo Y. Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis. J Vis Exp. 2020 Jul 16;(161). doi: 10.3791/61298. PMID: 32744521; PMCID: PMC8185428.
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J Vis Exp. 2020 Jul 16;(161). doi: 10.3791/61298.

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis.

Journal of visualized experiments : JoVE

Kyle G Kroeck, Weihua Qiu, Claudio Catalano, Thi Kim Hoang Trinh, Youzhong Guo

Affiliations

  1. Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University; Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy, Virginia Commonwealth University.
  2. Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University; Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy, Virginia Commonwealth University; [email protected].

PMID: 32744521 PMCID: PMC8185428 DOI: 10.3791/61298

Abstract

Protein-protein interactions in cell membrane systems play crucial roles in a wide range of biological processes- from cell-to-cell interactions to signal transduction; from sensing environmental signals to biological response; from metabolic regulation to developmental control. Accurate structural information of protein-protein interactions is crucial for understanding the molecular mechanisms of membrane protein complexes and for the design of highly specific molecules to modulate these proteins. Many in vivo and in vitro approaches have been developed for the detection and analysis of protein-protein interactions. Among them the structural biology approach is unique in that it can provide direct structural information of protein-protein interactions at the atomic level. However, current membrane protein structural biology is still largely limited to detergent-based methods. The major drawback of detergent-based methods is that they often dissociate or denature membrane protein complexes once their native lipid bilayer environment is removed by detergent molecules. We have been developing a native cell membrane nanoparticle system for membrane protein structural biology. Here, we demonstrate the use of this system in the analysis of protein-protein interactions on the cell membrane with a case study of the oligomeric state of AcrB.

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