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Murabe K, Tsukamoto T, Aizawa T, et al. Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin. J Am Chem Soc. 2020;142(37):16023-16030doi: 10.1021/jacs.0c07264.
Murabe, K., Tsukamoto, T., Aizawa, T., Demura, M., & Kikukawa, T. (2020). Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin. Journal of the American Chemical Society, 142(37), 16023-16030. https://doi.org/10.1021/jacs.0c07264
Murabe, Keisuke, et al. "Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin." Journal of the American Chemical Society vol. 142,37 (2020): 16023-16030. doi: https://doi.org/10.1021/jacs.0c07264
Murabe K, Tsukamoto T, Aizawa T, Demura M, Kikukawa T. Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin. J Am Chem Soc. 2020 Sep 16;142(37):16023-16030. doi: 10.1021/jacs.0c07264. Epub 2020 Sep 06. PMID: 32844642.
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J Am Chem Soc. 2020 Sep 16;142(37):16023-16030. doi: 10.1021/jacs.0c07264. Epub 2020 Sep 06.

Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin.

Journal of the American Chemical Society

Keisuke Murabe, Takashi Tsukamoto, Tomoyasu Aizawa, Makoto Demura, Takashi Kikukawa

Affiliations

  1. Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.
  2. Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan.
  3. Global Station for Soft Matter, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo 001-0021, Japan.

PMID: 32844642 DOI: 10.1021/jacs.0c07264

Abstract

For membrane transporters, substrate uptake and release reactions are major events during their transport cycles. Despite the functional importance of these events, it is difficult to identify their relevant structural intermediates because of the requirements of the experimental methods, which are to detect the timing of the formation and decay of intermediates and to detect the timing of substrate uptake and release. We report successfully achieving this for the light-driven Na

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