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Yonar M, Uehara M, Banouni N, et al. Cellular Mechanisms of Rejection of Optic and Sciatic Nerve Transplants: An Observational Study. Transplant Direct. 2020;6(8):e589doi: 10.1097/TXD.0000000000001012.
Yonar, M., Uehara, M., Banouni, N., Kasinath, V., Li, X., Jiang, L., Zhao, J., Bei, F., Shin, S. R., Cetrulo, C. L., Annabi, N., & Abdi, R. (2020). Cellular Mechanisms of Rejection of Optic and Sciatic Nerve Transplants: An Observational Study. Transplantation direct, 6(8), e589. https://doi.org/10.1097/TXD.0000000000001012
Yonar, Merve, et al. "Cellular Mechanisms of Rejection of Optic and Sciatic Nerve Transplants: An Observational Study." Transplantation direct vol. 6,8 (2020): e589. doi: https://doi.org/10.1097/TXD.0000000000001012
Yonar M, Uehara M, Banouni N, Kasinath V, Li X, Jiang L, Zhao J, Bei F, Shin SR, Cetrulo CL, Annabi N, Abdi R. Cellular Mechanisms of Rejection of Optic and Sciatic Nerve Transplants: An Observational Study. Transplant Direct. 2020 Jul 24;6(8):e589. doi: 10.1097/TXD.0000000000001012. eCollection 2020 Aug. PMID: 32766437; PMCID: PMC7382554.
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Transplant Direct. 2020 Jul 24;6(8):e589. doi: 10.1097/TXD.0000000000001012. eCollection 2020 Aug.

Cellular Mechanisms of Rejection of Optic and Sciatic Nerve Transplants: An Observational Study.

Transplantation direct

Merve Yonar, Mayuko Uehara, Naima Banouni, Vivek Kasinath, Xiaofei Li, Liwei Jiang, Jing Zhao, Fengfeng Bei, Su Ryon Shin, Curtis L Cetrulo, Nasim Annabi, Reza Abdi

Affiliations

  1. Transplantation Research Center, Renal Division, Brigham and Women's Hospital/Harvard Medical School, Boston, MA.
  2. Department of Neurosurgery, Brigham and Women's Hospital/Harvard Medical School, Cambridge, MA.
  3. Division of Engineering in Medicine, Brigham and Women's Hospital/Harvard Medical School, Boston, MA.
  4. Department of Surgery, Massachusetts General Hospital/Harvard Medical School, Boston, MA.
  5. Chemical and Biomolecular Engineering Department and Center for Minimally Invasive Therapeutics (C-MIT), California NanoSystems Institute (CNSI), University of California-Los Angeles, Los Angeles, CA.

PMID: 32766437 PMCID: PMC7382554 DOI: 10.1097/TXD.0000000000001012

Abstract

BACKGROUND: Organ transplantation is a standard therapeutic strategy for irreversible organ damage, but the utility of nerve transplantation remains generally unexplored, despite its potential benefit to a large patient population. Here, we aimed to establish a feasible preclinical mouse model for understanding the cellular mechanisms behind the rejection of peripheral and optic nerves.

METHODS: We performed syngenic and allogenic transplantation of optic and sciatic nerves in mice by inserting the nerve grafts inside the kidney capsule, and we assessed the allografts for signs of rejection through 14 d following transplantation. Then, we assessed the efficacy of CTLA4 Ig, Rapamycin, and anti-CD3 antibody in suppressing immune cell infiltration of the nerve allografts.

RESULTS: By 3 d posttransplantation, both sciatic and optic nerves transplanted from BALB/c mice into C57BL/6J recipients contained immune cell infiltrates, which included more CD11b

CONCLUSIONS: These findings establish the feasibility of a preclinical allogenic nerve transplantation model and provide the basis for future testing of directed, high-intensity immunosuppression in these mice.

Copyright © 2020 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.

Conflict of interest statement

The authors declare no conflicts of interest.

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