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Wang YT, Shi T, Srivastava S, et al. Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer. Cancers (Basel). 2020;12(9)doi: 10.3390/cancers12092335.
Wang, Y. T., Shi, T., Srivastava, S., Kagan, J., Liu, T., & Rodland, K. D. (2020). Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer. Cancers, 12(9), . https://doi.org/10.3390/cancers12092335
Wang, Yi-Ting, et al. "Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer." Cancers vol. 12,9 (2020). doi: https://doi.org/10.3390/cancers12092335
Wang YT, Shi T, Srivastava S, Kagan J, Liu T, Rodland KD. Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer. Cancers (Basel). 2020 Aug 19;12(9). doi: 10.3390/cancers12092335. PMID: 32825017; PMCID: PMC7564640.
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Cancers (Basel). 2020 Aug 19;12(9). doi: 10.3390/cancers12092335.

Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer.

Cancers

Yi-Ting Wang, Tujin Shi, Sudhir Srivastava, Jacob Kagan, Tao Liu, Karin D Rodland

Affiliations

  1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
  2. Cancer Biomarkers Research Group, Division of Cancer Prevention, National Cancer Institute, Bethesda, MD 20892, USA.
  3. Department of Cell, Developmental, and Cancer Biology, Oregon Health and Science University, Portland, OR 97201, USA.

PMID: 32825017 PMCID: PMC7564640 DOI: 10.3390/cancers12092335

Abstract

Extracellular vesicles (EVs) are released by nearly all cell types as part of normal cell physiology, transporting biological cargo, including nucleic acids and proteins, across the cell membrane. In pathological states such as cancer, EV-derived cargo may mirror the altered state of the cell of origin. Exosomes are the smaller, 50-150 nanometer-sized EVs released from fusion of multivesicular endosomes with the plasma membrane. Exosomes play important roles in cell-cell communication and participate in multiple cancer processes, including invasion and metastasis. Therefore, proteomic analysis of exosomes is a promising approach to discover potential cancer biomarkers, even though it is still at an early stage. Herein, we critically review the advances in exosome isolation methods and their compatibility with mass spectrometry (MS)-based proteomic analysis, as well as studies of exosomes in pathogenesis and progression of prostate and bladder cancer, two common urologic cancers whose incidence rates continue to rise annually. As urological tumors, both urine and blood samples are feasible for noninvasive or minimally invasive analysis. A better understanding of the biological cargo and functions of exosomes via high-throughput proteomics will help provide new insights into complex alterations in cancer and provide potential therapeutic targets and personalized treatment for patients.

Keywords: bladder cancer; exosome; exosome isolation method; prostate cancer; proteomics

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