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Danquah KO, Mensah K, Nkansah C, et al. Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients?. J Trop Med. 2020;2020:5194287doi: 10.1155/2020/5194287.
Danquah, K. O., Mensah, K., Nkansah, C., Appiah, S. K., Noagbe, M., Hardy, Y., Ntiamoah, D. O., Boateng, L. A., Annani-Akollor, M. E., Owiredu, E. W., Debrah, A. Y., & Addai-Mensah, O. (2020). Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients?. Journal of tropical medicine, 20205194287. https://doi.org/10.1155/2020/5194287
Danquah, Kwabena Owusu, et al. "Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients?." Journal of tropical medicine vol. 2020 (2020): 5194287. doi: https://doi.org/10.1155/2020/5194287
Danquah KO, Mensah K, Nkansah C, Appiah SK, Noagbe M, Hardy Y, Ntiamoah DO, Boateng LA, Annani-Akollor ME, Owiredu EW, Debrah AY, Addai-Mensah O. Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients?. J Trop Med. 2020 Aug 01;2020:5194287. doi: 10.1155/2020/5194287. eCollection 2020. PMID: 32802082; PMCID: PMC7416277.
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J Trop Med. 2020 Aug 01;2020:5194287. doi: 10.1155/2020/5194287. eCollection 2020.

Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients?.

Journal of tropical medicine

Kwabena Owusu Danquah, Kofi Mensah, Charles Nkansah, Samuel Kwasi Appiah, Mark Noagbe, Yasmine Hardy, David O Ntiamoah, Lillian Antwi Boateng, Max Efui Annani-Akollor, Eddie-Williams Owiredu, Alexander Yaw Debrah, Otchere Addai-Mensah

Affiliations

  1. Department of Medical Diagnostics, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  2. Department of Haematology, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
  3. HIV Clinic, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
  4. Department of Basic and Applied Biology, University of Energy and Natural Resource, Sunyani, Ghana.
  5. Department of Molecular Medicine, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

PMID: 32802082 PMCID: PMC7416277 DOI: 10.1155/2020/5194287

Abstract

This descriptive, cross-sectional study aimed at evaluating the prevalence of G6PD deficiency and the 376A ⟶ G, 202G ⟶ A single nucleotide polymorphisms (SNPs) among HIV patients attending care at a teaching hospital in Ghana and determine how the SNPs affect haematological profile in HIV. A total of 200 HIV-positive Ghanaians were recruited. Venous blood samples were obtained and complete blood count, and G6PD screening and genotyping for the 376A ⟶ G, 202G ⟶ A SNPs were performed. Out of the 200 participants, 13.0% (26/200) were G6PD-deficient based on the methemoglobin reductase technique, with 1.5% (3/200) and 11.5% (23/200) presenting with partial and full enzyme defect, respectively. Among the 13.0% participants with G6PD deficiency, 19.2% (5/26), 30.8% (8/26), and 19.2% (5/26) presented with 376A ⟶ G only (enzyme activity (EA): 1.19 U/g Hb), 202G ⟶A only (EA: 1.41 U/g Hb), and G202/A376 SNPs (EA: 1.14 U/g Hb), respectively. Having the 376A ⟶ G mutation was associated not only with lower red blood cell (RBC) count (3.38 × 10

Copyright © 2020 Kwabena Owusu Danquah et al.

Conflict of interest statement

The authors declare that they have no conflicts of interest.

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