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Armistead B, Quach P, Snyder JM, et al. Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection. J Infect Dis. 2021;223(8):1488-1496doi: 10.1093/infdis/jiaa548.
Armistead, B., Quach, P., Snyder, J. M., Santana-Ufret, V., Furuta, A., Brokaw, A., & Rajagopal, L. (2021). Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection. The Journal of infectious diseases, 223(8), 1488-1496. https://doi.org/10.1093/infdis/jiaa548
Armistead, Blair, et al. "Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection." The Journal of infectious diseases vol. 223,8 (2021): 1488-1496. doi: https://doi.org/10.1093/infdis/jiaa548
Armistead B, Quach P, Snyder JM, Santana-Ufret V, Furuta A, Brokaw A, Rajagopal L. Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection. J Infect Dis. 2021 Apr 23;223(8):1488-1496. doi: 10.1093/infdis/jiaa548. PMID: 32861213; PMCID: PMC8064051.
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J Infect Dis. 2021 Apr 23;223(8):1488-1496. doi: 10.1093/infdis/jiaa548.

Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection.

The Journal of infectious diseases

Blair Armistead, Phoenicia Quach, Jessica M Snyder, Verónica Santana-Ufret, Anna Furuta, Alyssa Brokaw, Lakshmi Rajagopal

Affiliations

  1. Department of Global Health, University of Washington, Seattle, Washington, USA.
  2. Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington, USA.
  3. Department of Comparative Medicine, University of Washington School of Medicine, Seattle, Washington, USA.
  4. Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.

PMID: 32861213 PMCID: PMC8064051 DOI: 10.1093/infdis/jiaa548

Abstract

BACKGROUND: Group B streptococci (GBS) are β-hemolytic, Gram-positive bacteria associated with fetal injury, preterm birth, spontaneous abortion, and neonatal infections. A key factor promoting GBS virulence is the β-hemolysin/cytolysin, a pigmented ornithine rhamnolipid (also known as granadaene) associated with the bacterial surface.

METHODS: A previous study indicated that GBS produce small structures known as membrane vesicles (MVs), which contain virulence-associated proteins. In this study, we show that GBS MVs are pigmented and hemolytic, indicating that granadaene is functionally active in MVs.

RESULTS: In addition, MVs from hyperhemolytic GBS induced greater cell death of neutrophils, T cells, and B cells compared with MVs from isogenic nonhemolytic GBS, implicating MVs as a potential mechanism for granadaene-mediated virulence. Finally, hemolytic MVs reduced oxidative killing of GBS and aggravated morbidity and mortality of neonatal mice infected with GBS.

CONCLUSIONS: These studies, taken together, reveal a novel mechanism by which GBS deploy a crucial virulence factor to promote bacterial dissemination and pathogenesis.

© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

Keywords: granadaene; group B streptococcus; hemolysin; immune evasion; membrane vesicles

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