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Vielh P, Balogh Z, Suciu V, et al. DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms. Cancers (Basel). 2020;12(9)doi: 10.3390/cancers12092529.
Vielh, P., Balogh, Z., Suciu, V., Richon, C., Job, B., Meurice, G., Valent, A., Lacroix, L., Marty, V., Motte, N., Dessen, P., Caillou, B., Ghuzlan, A. A., Bidart, J. M., Lazar, V., Hofman, P., Scoazec, J. Y., El-Naggar, A. K., & Schlumberger, M. (2020). DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms. Cancers, 12(9), . https://doi.org/10.3390/cancers12092529
Vielh, Philippe, et al. "DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms." Cancers vol. 12,9 (2020). doi: https://doi.org/10.3390/cancers12092529
Vielh P, Balogh Z, Suciu V, Richon C, Job B, Meurice G, Valent A, Lacroix L, Marty V, Motte N, Dessen P, Caillou B, Ghuzlan AA, Bidart JM, Lazar V, Hofman P, Scoazec JY, El-Naggar AK, Schlumberger M. DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms. Cancers (Basel). 2020 Sep 06;12(9). doi: 10.3390/cancers12092529. PMID: 32899953; PMCID: PMC7564487.
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Cancers (Basel). 2020 Sep 06;12(9). doi: 10.3390/cancers12092529.

DNA FISH Diagnostic Assay on Cytological Samples of Thyroid Follicular Neoplasms.

Cancers

Philippe Vielh, Zsofia Balogh, Voichita Suciu, Catherine Richon, Bastien Job, Guillaume Meurice, Alexander Valent, Ludovic Lacroix, Virginie Marty, Nelly Motte, Philippe Dessen, Bernard Caillou, Abir Al Ghuzlan, Jean-Michel Bidart, Vladimir Lazar, Paul Hofman, Jean-Yves Scoazec, Adel K El-Naggar, Martin Schlumberger

Affiliations

  1. Department of Medical Biology and Pathology, Gustave Roussy, Université Paris-Saclay and Experimental and Translational Pathology Platform, CNRS UMS3655-INSERM US23 AMMICA, 94805 Villejuif, France.
  2. Laboratory of Clinical and Experimental Pathology and Biobank, Pasteur Hospital, 06002 Nice, France.
  3. Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
  4. Department of Endocrinology, Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France.

PMID: 32899953 PMCID: PMC7564487 DOI: 10.3390/cancers12092529

Abstract

Although fine-needle aspiration cytology (FNAC) is helpful in determining whether thyroid nodules are benign or malignant, this distinction remains a cytological challenge in follicular neoplasms. Identification of genomic alterations in cytological specimens with direct and routine techniques would therefore have great clinical value. A series of 153 cases consisting of 72 and 81 histopathologically confirmed classic follicular adenomas (cFAs) and classic follicular thyroid carcinomas (cFTCs), respectively, was studied by means of different molecular techniques in three different cohorts of patients (pts). In the first cohort (training set) of 66 pts, three specific alterations characterized by array comparative genomic hybridization (aCGH) were exclusively found in half of cFTCs. These structural abnormalities corresponded to losses of 1p36.33-35.1 and 22q13.2-13.31, and gain of whole chromosome X. The second independent cohort (validation set) of 60 pts confirmed these data on touch preparations of frozen follicular neoplasms by triple DNA fluorescent in situ hybridization using selected commercially available probes. The third cohort, consisting of 27 archived cytological samples from an equal number of pts that had been obtained for preoperative FNAC and morphologically classified as and histologically verified to be follicular neoplasms, confirmed our previous findings and showed the feasibility of the DNA FISH (DNA fluorescent in situ hybridization) assay. All together, these data suggest that our triple DNA FISH diagnostic assay may detect 50% of cFTCs with a specificity higher than 98% and be useful as a low-cost adjunct to cytomorphology to help further classify follicular neoplasms on already routinely stained cytological specimens.

Keywords: array comparative genomic hybridization; fine-needle aspiration; fluorescent in situ hybridization; follicular neoplasms; indeterminate cytology; thyroid

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