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Thent ZC, Froemming GRA, Ismail ABM, et al. Phytoestrogens by inhibiting the non-classical oestrogen receptor, overcome the adverse effect of bisphenol A on hFOB 1.19 cells. Iran J Basic Med Sci. 2020;23(9):1155-1163doi: 10.22038/ijbms.2020.45296.10545.
Thent, Z. C., Froemming, G. R. A., Ismail, A. B. M., Fuad, S. B. S. A., & Muid, S. (2020). Phytoestrogens by inhibiting the non-classical oestrogen receptor, overcome the adverse effect of bisphenol A on hFOB 1.19 cells. Iranian journal of basic medical sciences, 23(9), 1155-1163. https://doi.org/10.22038/ijbms.2020.45296.10545
Thent, Zar Chi, et al. "Phytoestrogens by inhibiting the non-classical oestrogen receptor, overcome the adverse effect of bisphenol A on hFOB 1.19 cells." Iranian journal of basic medical sciences vol. 23,9 (2020): 1155-1163. doi: https://doi.org/10.22038/ijbms.2020.45296.10545
Thent ZC, Froemming GRA, Ismail ABM, Fuad SBSA, Muid S. Phytoestrogens by inhibiting the non-classical oestrogen receptor, overcome the adverse effect of bisphenol A on hFOB 1.19 cells. Iran J Basic Med Sci. 2020 Sep;23(9):1155-1163. doi: 10.22038/ijbms.2020.45296.10545. PMID: 32963737; PMCID: PMC7491500.
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Iran J Basic Med Sci. 2020 Sep;23(9):1155-1163. doi: 10.22038/ijbms.2020.45296.10545.

Phytoestrogens by inhibiting the non-classical oestrogen receptor, overcome the adverse effect of bisphenol A on hFOB 1.19 cells.

Iranian journal of basic medical sciences

Zar Chi Thent, Gabriele Ruth Anisah Froemming, Aletza Binti Mohd Ismail, Syed Baharom Syed Ahmad Fuad, Suhaila Muid

Affiliations

  1. Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000 Selangor, Malaysia.
  2. Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak (UNIMAS), 94300 Kota Samarahan, Sarawak, Malaysia.
  3. Institute for Pathology, Laboratory and Forensic Medicine (IPPerForM), Universiti Teknologi MARA, Sungai Buloh Campus, 47000 Selangor, Malaysia.

PMID: 32963737 PMCID: PMC7491500 DOI: 10.22038/ijbms.2020.45296.10545

Abstract

OBJECTIVES: Since bisphenol A (BPA) induces bone loss and phytoestrogens enhance the osteoblastogenesis by binding to the non-classical and classical oestrogen receptors, respectively, the present study was aimed to observe the osteoprotective effect of phytoestrogens on BPA-induced osteoblasts in hFOB 1.19 cells.

MATERIALS AND METHODS: All groups of hFOB 1.19 cells were induced with 12.5 μg/ml of BPA except the control (Ctrl) group. Meanwhile, treated groups received phytoestrogens; Daidzein (Dz), Genistein (Gt), Equol (Eq) and 17β-oestradiol (Est) in different concentrations for 24 hr duration.

RESULTS: We found that the protein expression of non-classical oestrogen-related receptor (ERRG) was highly expressed in BPA group, whereas classical oestrogen receptor alpha (ERα) and oestrogen receptor beta (ERβ) were relatively increased with phytoestrogens treatment under BPA exposure. The dense actin cytoskeletal filaments were also observed. qRT-PCR showed up-regulation of mitogen-activated protein kinase 3 (MAPK3) and G protein-coupled receptor 30 (GPR30) expressions; significant down-regulation of ERRG and up-regulation of ERα and ERβ were observed in phytoestrogens-treated cells, which was supported by the increased expressions of oestrogen receptor 1 (ESR1) and oestrogen receptor 2 (ESR2).

CONCLUSION: Phytoestrogens improved the deteriorative effect of BPA via down-regulation of ERRG in hFOB 1.19 cells. This study showed that the efficacy of consumption of phytoestrogens in rendering them as potential therapeutic strategy in combating the adverse bone effects of BPA.

Keywords: Bisphenol A; ERRG; Oestrogens receptors; Phytoestrogens; hFOB 1.19 cells

References

  1. Life Sci. 2018 Oct 1;210:214-223 - PubMed
  2. Carcinogenesis. 1996 Feb;17(2):271-5 - PubMed
  3. Steroids. 2012 Feb;77(3):174-84 - PubMed
  4. Nutrition. 2013 Jan;29(1):250-7 - PubMed
  5. N Engl J Med. 1993 Oct 14;329(16):1141-6 - PubMed
  6. Growth Factors. 2006 Mar;24(1):21-44 - PubMed
  7. Biochem Biophys Res Commun. 2012 Jul 6;423(3):503-8 - PubMed
  8. Bone. 2007 Jun;40(6):1529-35 - PubMed
  9. Br J Nutr. 2014 Jan 14;111(1):55-63 - PubMed
  10. Eur J Nutr. 2008 Aug;47(5):273-9 - PubMed
  11. Stem Cells. 2007 Jan;25(1):125-31 - PubMed
  12. FASEB J. 2014 Jul;28(7):3124-33 - PubMed
  13. Endocr J. 2002 Aug;49(4):465-71 - PubMed
  14. J Steroid Biochem Mol Biol. 2009 Feb;113(3-5):281-9 - PubMed
  15. J Endocrinol. 2008 May;197(2):R1-6 - PubMed
  16. Int Immunopharmacol. 2016 Nov;40:32-40 - PubMed
  17. Am J Physiol Endocrinol Metab. 2009 Mar;296(3):E490-6 - PubMed
  18. J Biol Chem. 2009 May 22;284(21):14211-8 - PubMed
  19. Genes Dev. 2004 Feb 1;18(3):290-305 - PubMed
  20. Life Sci. 2013 May 30;92(20-21):993-1003 - PubMed
  21. J Bone Miner Res. 2002 Jan;17(1):101-10 - PubMed
  22. Biochem Biophys Res Commun. 2008 Aug 29;373(3):408-13 - PubMed
  23. Nutr Res. 2017 Jun;42:20-30 - PubMed
  24. Planta Med. 2009 Apr;75(5):459-65 - PubMed
  25. J Biol Chem. 2001 May 25;276(21):17808-14 - PubMed
  26. Toxicol Appl Pharmacol. 2015 Mar 1;283(2):139-46 - PubMed
  27. J Nutr Biochem. 2015 Dec;26(12):1491-501 - PubMed
  28. Biochem Biophys Res Commun. 2002 Jul 12;295(2):417-22 - PubMed
  29. Biochim Biophys Acta. 2007 May;1773(5):675-86 - PubMed
  30. Genet Mol Res. 2014 Jul 04;13(3):5055-63 - PubMed
  31. Life Sci. 2013 Sep 17;93(9-11):367-72 - PubMed
  32. Mol Cell Biol. 2009 Nov;29(21):5843-57 - PubMed
  33. Endocrinology. 1997 Mar;138(3):863-70 - PubMed
  34. J Biol Chem. 2008 Oct 10;283(41):27585-97 - PubMed
  35. J Clin Densitom. 2011 Jan-Mar;14(1):47-57 - PubMed
  36. J Nutr Biochem. 2012 Jan;23(1):27-38 - PubMed
  37. J Cell Biochem. 2001 Aug 21-Sep 5;83(3):448-62 - PubMed
  38. Mol Cell Endocrinol. 2012 May 22;355(2):201-7 - PubMed
  39. J Nutr. 2004 Oct;134(10):2623-7 - PubMed
  40. Life Sci. 2003 Sep 12;73(17):2237-47 - PubMed
  41. J Cell Physiol. 2006 Dec;209(3):786-801 - PubMed
  42. Environ Health Perspect. 1995 Oct;103 Suppl 7:113-22 - PubMed
  43. Arthritis Res Ther. 2009;11(5):241 - PubMed
  44. PLoS One. 2014 Oct 14;9(10):e109592 - PubMed
  45. Cancer Res. 1994 Jan 1;54(1):28-32 - PubMed
  46. Endocrinology. 2010 Oct;151(10):4926-37 - PubMed
  47. Clin Biochem. 2012 Dec;45(18):1602-6 - PubMed

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