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Goecke NB, Kobberø M, Kusk TK, et al. Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing. Porcine Health Manag. 2020;6:23doi: 10.1186/s40813-020-00161-3.
Goecke, N. B., Kobberø, M., Kusk, T. K., Hjulsager, C. K., Pedersen, K. S., Kristensen, C. S., & Larsen, L. E. (2020). Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing. Porcine health management, 623. https://doi.org/10.1186/s40813-020-00161-3
Goecke, Nicole B, et al. "Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing." Porcine health management vol. 6 (2020): 23. doi: https://doi.org/10.1186/s40813-020-00161-3
Goecke NB, Kobberø M, Kusk TK, Hjulsager CK, Pedersen KS, Kristensen CS, Larsen LE. Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing. Porcine Health Manag. 2020 Sep 08;6:23. doi: 10.1186/s40813-020-00161-3. eCollection 2020. PMID: 32922832; PMCID: PMC7476771.
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Porcine Health Manag. 2020 Sep 08;6:23. doi: 10.1186/s40813-020-00161-3. eCollection 2020.

Objective pathogen monitoring in nursery and finisher pigs by monthly laboratory diagnostic testing.

Porcine health management

Nicole B Goecke, Maja Kobberø, Thomas K Kusk, Charlotte K Hjulsager, Ken Steen Pedersen, Charlotte S Kristensen, Lars E Larsen

Affiliations

  1. Centre for Diagnostics, Technical University of Denmark, 2800 Kgs Lyngby, Denmark.
  2. Present address: University of Copenhagen, 1870 Frederiksberg C, Denmark.
  3. Present address: Statens Serum Institut, 2300 Copenhagen S, Denmark.
  4. Ø-Vet A/S, 4700 Næstved, Denmark.
  5. SEGES Danish Pig Research Centre, 1609 Copenhagen V, Denmark.

PMID: 32922832 PMCID: PMC7476771 DOI: 10.1186/s40813-020-00161-3

Abstract

BACKGROUND: Infectious diseases are of great economic importance in commercial pig production, causing both clinical and subclinical disease, with influence on welfare, productivity, and antibiotic use. The causes of these diseases are often multifactorial and laboratory diagnostics are seldom routinely performed. The aim of the present study was to explore the benefits of monthly pathogen monitoring in nursery and finisher herds and to examine association between laboratory results and observed clinical signs, including coughing and diarrhoea. Three monthly samplings were conducted in three different age groups in six nursery and four finisher production units. For each unit, two pens were randomly selected in each age group and evaluated for coughing and diarrhoea events. Furthermore, faecal sock and oral fluid samples were collected in the selected pens and analysed for 18 respiratory and enteric viral and bacterial pathogens using the high-throughput real-time PCR BioMark HD platform (Fluidigm, South San Francisco, USA).

RESULTS: In total, 174 pens were sampled in which eight coughing events and 77 diarrhoeic events were observed. The overall findings showed that swine influenza A virus, porcine circovirus 2, porcine cytomegalovirus,

CONCLUSIONS: The use of high-throughput real-time PCR analysis for continuous monitoring of pathogens and thereby dynamics of infections in a pig herd, provided the veterinarian and farmer with an objective knowledge on the distribution of pathogens in the herd. In addition, the use of a high-throughput method in combination with information about clinical signs, productivity, health status and antibiotic consumption, presents a new and innovative way of diagnosing and monitoring pig herds and even to a lower cost than the traditional method.

© The Author(s) 2020.

Keywords: Coughing; Diagnostics; Diarrhoea; Enteric bacteria; Enteric viruses; High-throughput real-time PCR; Monitoring; Respiratory bacteria; Respiratory viruses

Conflict of interest statement

Competing interestsNG is employed by the Technical University of Denmark that may benefit commercially by the use of the system described in the study. CK works for SEGES Danish Pig Research Centre. T

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