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Wang H, Shiveshwarkar P, Brzozowski R, et al. Innovative optofluidics and microscopy-based rapid analysis of pathogens. Biomed Opt Express. 2020;11(9):5060-5069doi: 10.1364/BOE.396345.
Wang, H., Shiveshwarkar, P., Brzozowski, R., Zhdanov, A., Shi, S., Eswara, P., & Pyayt, A. (2020). Innovative optofluidics and microscopy-based rapid analysis of pathogens. Biomedical optics express, 11(9), 5060-5069. https://doi.org/10.1364/BOE.396345
Wang, Hao, et al. "Innovative optofluidics and microscopy-based rapid analysis of pathogens." Biomedical optics express vol. 11,9 (2020): 5060-5069. doi: https://doi.org/10.1364/BOE.396345
Wang H, Shiveshwarkar P, Brzozowski R, Zhdanov A, Shi S, Eswara P, Pyayt A. Innovative optofluidics and microscopy-based rapid analysis of pathogens. Biomed Opt Express. 2020 Aug 17;11(9):5060-5069. doi: 10.1364/BOE.396345. eCollection 2020 Sep 01. PMID: 33014600; PMCID: PMC7510850.
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Biomed Opt Express. 2020 Aug 17;11(9):5060-5069. doi: 10.1364/BOE.396345. eCollection 2020 Sep 01.

Innovative optofluidics and microscopy-based rapid analysis of pathogens.

Biomedical optics express

Hao Wang, Priyanka Shiveshwarkar, Robert Brzozowski, Arseny Zhdanov, Shulin Shi, Prahathees Eswara, Anna Pyayt

Affiliations

  1. Department of Chemical and Biomedical Engineering, University of South Florida, 4202 E. Fowler Ave, ENB118, Tampa, FL 33620, USA.
  2. Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 E, Fowler Ave. ISA 2015, Tampa, FL 33620, USA.

PMID: 33014600 PMCID: PMC7510850 DOI: 10.1364/BOE.396345

Abstract

The timely knowledge and prescription of the most suitable antibiotic to treat bacterial infections is critical for the recovery of patients battling life-threatening bacterial infections. Unfortunately, current standard-of-care approaches relies on the empiric prescription of an antibiotic, as determination of the most effective antibiotic requires multiple time-consuming steps. These steps often include culturing of the bacterium responsible for infection and subsequent antibiotic susceptibility testing. Here we introduce an optofluidic technology that allows us to capture bacterial cells efficiently and rapidly from different biological samples and use the captured cells for rapid antibiotic selection thereby bypassing the need to culture the bacterium.

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Conflict of interest statement

The authors declare no conflicts of interest.

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