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Xu Y, Zhang T, Chen Y, et al. Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells. Stem Cells Int. 2020;2020:8894449doi: 10.1155/2020/8894449.
Xu, Y., Zhang, T., Chen, Y., Shi, Q., Li, M., Qin, T., Hu, J., Lu, H., Liu, J., & Chen, C. (2020). Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells. Stem cells international, 20208894449. https://doi.org/10.1155/2020/8894449
Xu, Yan, et al. "Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells." Stem cells international vol. 2020 (2020): 8894449. doi: https://doi.org/10.1155/2020/8894449
Xu Y, Zhang T, Chen Y, Shi Q, Li M, Qin T, Hu J, Lu H, Liu J, Chen C. Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells. Stem Cells Int. 2020 Sep 30;2020:8894449. doi: 10.1155/2020/8894449. eCollection 2020. PMID: 33061993; PMCID: PMC7545436.
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Stem Cells Int. 2020 Sep 30;2020:8894449. doi: 10.1155/2020/8894449. eCollection 2020.

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells.

Stem cells international

Yan Xu, Tao Zhang, Yang Chen, Qiang Shi, Muzhi Li, Tian Qin, Jianzhong Hu, Hongbin Lu, Jun Liu, Can Chen

Affiliations

  1. Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, China 410008.
  2. Hunan Engineering Research Center of Sports and Health, Changsha, China 410008.
  3. Xiangya Hospital-International Chinese Musculoskeletal Research Society Sports Medicine Research Centre, Changsha, China 410008.
  4. Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China 410008.
  5. Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China 410008.
  6. Department of Limbs (Foot and Hand) Microsurgery, Affiliated Chenzhou No.1 People's Hospital, Southern Medical University, Chenzhou, China 423000.
  7. Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China 410008.

PMID: 33061993 PMCID: PMC7545436 DOI: 10.1155/2020/8894449

Abstract

Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to bridge the gap from rodents to humans. In this study, we reported the isolation and characterization of urine-derived stem cells (USCs) from mature healthy beagle dogs. The isolated cells showed fibroblast-like morphology and had good clonogenicity and proliferation. Meanwhile, these cells positively expressed multiple markers of MSCs (CD29, CD44, CD90, and CD73), but negatively expressed for hematopoietic antigens (CD11b, CD34, and CD45). Additionally, after induction culturing, the isolated cells can be differentiated into osteogenic, adipogenic, chondrogenic, and tenogenic lineages. The successful isolation and verification of USCs from canine were useful for studying cell-based therapies and developing new treatments for musculoskeletal injuries using the preclinical canine model.

Copyright © 2020 Yan Xu et al.

Conflict of interest statement

The authors declare no competing financial interests.

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