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El Sayed Zaki M, Mashaly GE, Alsayed MAL, et al. Molecular study of human astrovirus in Egyptian children with acute gastroenteritis. Germs. 2020;10(4):167-173doi: 10.18683/germs.2020.1202.
El Sayed Zaki, M., Mashaly, G. E., Alsayed, M. A. L., & Nomir, M. M. (2020). Molecular study of human astrovirus in Egyptian children with acute gastroenteritis. Germs, 10(4), 167-173. https://doi.org/10.18683/germs.2020.1202
El Sayed Zaki, Maysaa, et al. "Molecular study of human astrovirus in Egyptian children with acute gastroenteritis." Germs vol. 10,4 (2020): 167-173. doi: https://doi.org/10.18683/germs.2020.1202
El Sayed Zaki M, Mashaly GE, Alsayed MAL, Nomir MM. Molecular study of human astrovirus in Egyptian children with acute gastroenteritis. Germs. 2020 Sep 01;10(4):167-173. doi: 10.18683/germs.2020.1202. eCollection 2020 Sep. PMID: 33134194; PMCID: PMC7572211.
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Germs. 2020 Sep 01;10(4):167-173. doi: 10.18683/germs.2020.1202. eCollection 2020 Sep.

Molecular study of human astrovirus in Egyptian children with acute gastroenteritis.

Germs

Maysaa El Sayed Zaki, Ghada El-Saeed Mashaly, Mona Abdel Latif Alsayed, Manal Mahmoud Nomir

Affiliations

  1. MD, Clinical Pathology Department, Mansoura Faculty of Medicine, Egypt.
  2. MD, Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt.
  3. MD, Lecturer of Pediatrics and Pediatric Gastroenterology and Hepatology, Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
  4. MD, Clinical Pathology, Students hospital, Mansoura University, Egypt.

PMID: 33134194 PMCID: PMC7572211 DOI: 10.18683/germs.2020.1202

Abstract

INTRODUCTION: Human astrovirus (HAstV) has been increasingly identified as an important cause of acute gastroenteritis in young children. Limited information is available about the prevalence and genotype distribution of classic HAstV causing acute gastroenteritis in Egyptian children.

METHODS: Stool samples were collected from 100 infants and children attending the gastroenterology outpatient clinic in Mansoura University Children Hospital and suffering from acute gastroenteritis during the period extending from January 2018 to January 2019. Samples were tested for HAstV using reverse transcription PCR. Genotyping was performed using type-specific reverse transcription nested PCR.

RESULTS: Among 100 children included in this study, the detection rate of HAstV was 11% (11 patients). There was a significant difference regarding age between cases positive and negative for HAstV (p=0.005). There was a higher prevalence of HAstV in children aged one year or younger. Significant association was detected between HAstV positive cases and rural residence (p=0.002), summer season (p=0.025) and fever (p=0.017). The HAstV genotypes detected were HAstV-8 (8/11, 72.7%), HAstV-3 (2/11, 18.2%) and HAstV-2 (1/11, 9.1%).

CONCLUSIONS: This study suggests that HAstV is a common pathogen causing gastroenteritis in Egyptian children especially in rural areas. The most frequent HAstV genotype in our study was HAstV-8.

GERMS.

Keywords: Astrovirus; children; diarrhea; gastroenteritis; genotypes

Conflict of interest statement

Conflicts of interest: All authors - none to declare.

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