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Park I, Yang S, Choi G, et al. An Integrated Approach for Efficient and Accurate Medicinal Cuscutae Semen Identification. Plants (Basel). 2020;9(11)doi: 10.3390/plants9111410.
Park, I., Yang, S., Choi, G., Moon, B. C., & Song, J. H. (2020). An Integrated Approach for Efficient and Accurate Medicinal Cuscutae Semen Identification. Plants (Basel, Switzerland), 9(11), . https://doi.org/10.3390/plants9111410
Park, Inkyu, et al. "An Integrated Approach for Efficient and Accurate Medicinal Cuscutae Semen Identification." Plants (Basel, Switzerland) vol. 9,11 (2020). doi: https://doi.org/10.3390/plants9111410
Park I, Yang S, Choi G, Moon BC, Song JH. An Integrated Approach for Efficient and Accurate Medicinal Cuscutae Semen Identification. Plants (Basel). 2020 Oct 22;9(11). doi: 10.3390/plants9111410. PMID: 33105814; PMCID: PMC7690581.
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Plants (Basel). 2020 Oct 22;9(11). doi: 10.3390/plants9111410.

An Integrated Approach for Efficient and Accurate Medicinal Cuscutae Semen Identification.

Plants (Basel, Switzerland)

Inkyu Park, Sungyu Yang, Goya Choi, Byeong Cheol Moon, Jun-Ho Song

Affiliations

  1. Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.

PMID: 33105814 PMCID: PMC7690581 DOI: 10.3390/plants9111410

Abstract

To guarantee the safety and efficacy of herbal medicines, accurate identification and quality evaluation are crucial. The ripe dried seeds of Cuscuta australis R.Br. and C. chinensis Lam. are known as Cuscutae Semen (CS) and are widely consumed in Northeast Asia; however, the seeds of other species can be misidentified as CS owing to morphological similarities, leading to misuse. In this report, we propose a multilateral strategy combining microscopic techniques with statistical analysis and DNA barcoding using a genus-specific primer to facilitate the identification and authentication of CS. Morphology-based identification using microscopy revealed that the useful diagnostic characteristics included general shape, embryo exudation, hairiness, and testa ornamentation, which were used to develop an effective identification key. In addition, we conducted DNA barcoding-based identification to ensure accurate authentication. A novel DNA barcode primer was produced from the chloroplast

Keywords: Cuscuta chinensis; Cuscutae Semen; barcode primer; endosperm; light microscope; morphological identification key; rbcL gene; scanning electron microscope; seed morphology

Conflict of interest statement

The authors declare that there are no conflicts of interest.

References

  1. J Ethnopharmacol. 2020 Apr 24;252:112574 - PubMed
  2. Nat Methods. 2012 Jul 30;9(8):772 - PubMed
  3. Phytochem Anal. 2018 Mar;29(2):123-128 - PubMed
  4. BMC Plant Biol. 2007 Aug 22;7:45 - PubMed
  5. Sci Rep. 2016 Jan 07;6:18723 - PubMed
  6. J Ethnopharmacol. 1986 Oct;18(1):21-31 - PubMed
  7. Int J Mol Sci. 2019 Jun 03;20(11): - PubMed
  8. Int J Mol Sci. 2016 Dec 07;17(12): - PubMed
  9. Am J Bot. 2009 Sep;96(9):1744-50 - PubMed
  10. Syst Biol. 2012 May;61(3):539-42 - PubMed
  11. BMC Med. 2013 Oct 11;11:222 - PubMed
  12. Oncotarget. 2016 Jun 14;7(24):35680-35691 - PubMed
  13. Front Pharmacol. 2020 Jun 09;11:876 - PubMed
  14. Plant Biotechnol J. 2016 Jan;14(1):8-21 - PubMed
  15. Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W273-9 - PubMed
  16. Biotechnol Adv. 2014 Nov 15;32(7):1237-1244 - PubMed
  17. J Ethnopharmacol. 2012 Apr 10;140(3):513-8 - PubMed
  18. Mol Biol Evol. 2017 Dec 1;34(12):3299-3302 - PubMed
  19. Asian J Androl. 2000 Jun;2(2):99-102 - PubMed
  20. Front Pharmacol. 2019 Oct 24;10:1227 - PubMed
  21. Evid Based Complement Alternat Med. 2014;2014:150153 - PubMed
  22. Planta Med. 2002 Mar;68(3):237-43 - PubMed
  23. Planta Med. 2016 Sep;82(14):1225-35 - PubMed
  24. Ann Acad Med Singap. 2006 Nov;35(11):764-9 - PubMed
  25. Avicenna J Phytomed. 2012 Spring;2(2):105-12 - PubMed
  26. Biol Pharm Bull. 2007 Aug;30(8):1427-31 - PubMed
  27. Front Plant Sci. 2019 Feb 05;10:68 - PubMed
  28. J Ethnopharmacol. 2007 Apr 20;111(1):123-8 - PubMed
  29. Food Chem Toxicol. 2008 May;46(5):1771-7 - PubMed
  30. Nephrol Dial Transplant. 2007 Jun;22(6):1512-7 - PubMed
  31. Mol Biol Evol. 2013 Dec;30(12):2725-9 - PubMed
  32. Methods Find Exp Clin Pharmacol. 2003 Jul-Aug;25(6):447-52 - PubMed
  33. Nutrients. 2019 Dec 12;11(12): - PubMed
  34. Planta Med. 2011 Nov;77(17):1950-7 - PubMed
  35. Vet Hum Toxicol. 1995 Dec;37(6):562-6 - PubMed
  36. J Ethnopharmacol. 2005 May 13;99(1):99-103 - PubMed
  37. BMC Plant Biol. 2007 Oct 24;7:57 - PubMed
  38. Immunobiology. 2011 Oct;216(10):1103-9 - PubMed
  39. Plants (Basel). 2020 Apr 29;9(5): - PubMed

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