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Schnepf M, von Reutern M, Ludwig C, et al. Transcription Factor Binding Affinities and DNA Shape Readout. iScience. 2020;23(11):101694doi: 10.1016/j.isci.2020.101694.
Schnepf, M., von Reutern, M., Ludwig, C., Jung, C., & Gaul, U. (2020). Transcription Factor Binding Affinities and DNA Shape Readout. iScience, 23(11), 101694. https://doi.org/10.1016/j.isci.2020.101694
Schnepf, Max, et al. "Transcription Factor Binding Affinities and DNA Shape Readout." iScience vol. 23,11 (2020): 101694. doi: https://doi.org/10.1016/j.isci.2020.101694
Schnepf M, von Reutern M, Ludwig C, Jung C, Gaul U. Transcription Factor Binding Affinities and DNA Shape Readout. iScience. 2020 Oct 15;23(11):101694. doi: 10.1016/j.isci.2020.101694. eCollection 2020 Nov 20. PMID: 33163946; PMCID: PMC7607496.
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iScience. 2020 Oct 15;23(11):101694. doi: 10.1016/j.isci.2020.101694. eCollection 2020 Nov 20.

Transcription Factor Binding Affinities and DNA Shape Readout.

iScience

Max Schnepf, Marc von Reutern, Claudia Ludwig, Christophe Jung, Ulrike Gaul

Affiliations

  1. Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 München, Germany.

PMID: 33163946 PMCID: PMC7607496 DOI: 10.1016/j.isci.2020.101694

Abstract

An essential event in gene regulation is the binding of a transcription factor (TF) to its target DNA. Models considering the interactions between the TF and the DNA geometry proved to be successful approaches to describe this binding event, while conserving data interpretability. However, a direct characterization of the DNA shape contribution to binding is still missing due to the lack of accurate and large-scale binding affinity data. Here, we use a binding assay we recently established to measure with high sensitivity the binding specificities of 13

© 2020 The Authors.

Keywords: Biomolecules; Molecular Biology; Molecular Mechanism of Gene Regulation

Conflict of interest statement

The authors declare no conflict of interests.

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