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Ismail SN, Ghani NSA, Ab Razak SF, et al. Genetic Diversity of Pineapple (. Trop Life Sci Res. 2020;31(3):15-27doi: 10.21315/tlsr2020.31.3.2.
Ismail, S. N., Ghani, N. S. A., Ab Razak, S. F., Abidin, R. A. Z., Mohd Yusof, M. F., Zubir, M. N., & Zainol, R. (2020). Genetic Diversity of Pineapple (. Tropical life sciences research, 31(3), 15-27. https://doi.org/10.21315/tlsr2020.31.3.2
Ismail, Siti Norhayati, et al. "Genetic Diversity of Pineapple (." Tropical life sciences research vol. 31,3 (2020): 15-27. doi: https://doi.org/10.21315/tlsr2020.31.3.2
Ismail SN, Ghani NSA, Ab Razak SF, Abidin RAZ, Mohd Yusof MF, Zubir MN, Zainol R. Genetic Diversity of Pineapple (. Trop Life Sci Res. 2020 Oct;31(3):15-27. doi: 10.21315/tlsr2020.31.3.2. Epub 2020 Oct 15. PMID: 33214853; PMCID: PMC7652243.
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Trop Life Sci Res. 2020 Oct;31(3):15-27. doi: 10.21315/tlsr2020.31.3.2. Epub 2020 Oct 15.

Genetic Diversity of Pineapple (.

Tropical life sciences research

Siti Norhayati Ismail, Nurul Shamimi Abdul Ghani, Shahril Firdaus Ab Razak, Rabiatul Adawiah Zainal Abidin, Muhammad Fairuz Mohd Yusof, Mohd Nizam Zubir, Rozlaily Zainol

Affiliations

  1. Biotechnology and Nanotechnology Research Centre, Malaysian Agriculture Research and Development Institute (MARDI) Headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia.
  2. Horticulture Research Centre, MARDI Pontian, KM 53, Jalan Johor, 82000 Pontian, Johor, Malaysia.
  3. Industrial Crop Research Centre, Malaysian Agricultural Research and Development Institute (MARDI) Headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia.

PMID: 33214853 PMCID: PMC7652243 DOI: 10.21315/tlsr2020.31.3.2

Abstract

Assessments of genetic diversity have been claimed to be significantly efficient in utilising and managing resources of genetic for breeding programme. In this study, variations in genetic were observed in 65 pineapple accessions gathered from germplasm available at Malaysian Agriculture Research and Development Institute (MARDI) located in Pontian, Johor via 15 markers of simple sequence repeat (SSR). The results showed that 59 alleles appeared to range from 2.0 to 6.0 alleles with a mean of 3.9 alleles per locus, thus displaying polymorphism for all samples at a moderate level. Furthermore, the values of polymorphic information content (PIC) had been found to range between 0.104 (TsuAC035) and 0.697 (Acom_9.9), thus averaging at the value of 0.433. In addition, the expected and the observed heterozygosity of each locus seemed to vary within the ranges of 0.033 to 0.712, and from 0.033 to 0.885, along with the average values of 0.437 and 0.511, respectively. The population structure analysis via method of delta K (ΔK), along with mean of L (K) method, revealed that individuals from the germplasm could be divided into two major clusters based on genetics (K = 2), namely Group 1 and Group 2. As such, five accessions (Yankee, SRK Chalok, SCK Giant India, SC KEW5 India and SC1 Thailand) were clustered in Group 1, while the rest were clustered in Group 2. These outcomes were also supported by the dendrogram, which had been generated through the technique of unweighted pair group with arithmetic mean (UPGMA). These analyses appear to be helpful amongst breeders to maintain and to manage their collections of germplasm. Besides, the data gathered in this study can be useful for breeders to exploit the area of genetic diversity in estimating the level of heterosis.

© Penerbit Universiti Sains Malaysia, 2020.

Keywords: Co-dominant; Germplasm Characterisation; Molecular Marker; Simple Sequence Repeat (SSR)

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