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Vink HA, Versnel H, Kroon S, et al. BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies. Hear Res. 2020;400:108114doi: 10.1016/j.heares.2020.108114.
Vink, H. A., Versnel, H., Kroon, S., Klis, S. F. L., & Ramekers, D. (2021). BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies. Hearing research, 400108114. https://doi.org/10.1016/j.heares.2020.108114
Vink, Henk A, et al. "BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies." Hearing research vol. 400 (2021): 108114. doi: https://doi.org/10.1016/j.heares.2020.108114
Vink HA, Versnel H, Kroon S, Klis SFL, Ramekers D. BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies. Hear Res. 2021 Feb;400:108114. doi: 10.1016/j.heares.2020.108114. Epub 2020 Nov 18. PMID: 33271438.
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Hear Res. 2021 Feb;400:108114. doi: 10.1016/j.heares.2020.108114. Epub 2020 Nov 18.

BDNF-mediated preservation of spiral ganglion cell peripheral processes and axons in comparison to that of their cell bodies.

Hearing research

Henk A Vink, Huib Versnel, Steven Kroon, Sjaak F L Klis, Dyan Ramekers

Affiliations

  1. Department of Otorhinolaryngology and Head & Neck Surgery, University Medical Center Utrecht, Utrecht University, Room G.02.531, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands; UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands. Electronic address: [email protected].
  2. Department of Otorhinolaryngology and Head & Neck Surgery, University Medical Center Utrecht, Utrecht University, Room G.02.531, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands; UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands. Electronic address: [email protected].
  3. Department of Otorhinolaryngology and Head & Neck Surgery, University Medical Center Utrecht, Utrecht University, Room G.02.531, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands.
  4. Department of Otorhinolaryngology and Head & Neck Surgery, University Medical Center Utrecht, Utrecht University, Room G.02.531, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands; UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands. Electronic address: [email protected].
  5. Department of Otorhinolaryngology and Head & Neck Surgery, University Medical Center Utrecht, Utrecht University, Room G.02.531, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands; UMC Utrecht Brain Center, Utrecht University, Utrecht, the Netherlands. Electronic address: [email protected].

PMID: 33271438 DOI: 10.1016/j.heares.2020.108114

Abstract

Treatment with neurotrophins prevents degeneration of spiral ganglion cells (SGCs) after severe hair cell loss. In a previous study we demonstrated a long-lasting effect with brain-derived neurotrophic factor (BDNF) after cessation of treatment. In that study the survival of the SGC cell bodies was examined. Here we address the question whether their peripheral processes and central processes (axons) were protected by this treatment as well in the cochleas of the aforementioned study. Guinea pigs were deafened by co-administration of kanamycin and furosemide. Two weeks after deafening the right cochleas were implanted with an intracochlear electrode array combined with a cannula connected to an osmotic pump filled with BDNF solution. Four weeks later the treatment was stopped by surgically removing the osmotic pump. At that point, or another four or eight weeks later, the animals were sacrificed for histological analysis. Control groups consisted of normal-hearing animals, and three groups of deafened animals: two-weeks-deaf untreated animals, and six- and fourteen-weeks-deaf sham-treated animals. Cochleas were processed for analysis of: (1) the myelinated portion of peripheral processes in the osseous spiral lamina, (2) the cell bodies in Rosenthal's canal, and (3) axons in the internal acoustic meatus. Packing densities and cross-sectional areas were determined using light microscopy. Up to eight weeks after treatment cessation the numbers of peripheral processes and axons were significantly higher than in untreated cochleas of control animals. Whereas the numbers of cell bodies and axons were similar to those at the start of treatment, the peripheral processes were significantly less well preserved. This smaller protective effect was found mainly in the apical turns. Strategies to prevent SGC degeneration after hair cell loss should consider the differential effects on the various neural elements.

Copyright © 2020. Published by Elsevier B.V.

Keywords: Auditory nerve; Cochlea; Guinea pig; Hearing loss; Neurodegeneration; Neuroprotection; Neurotrophic factor

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