Front Microbiol. 2020 Nov 03;11:584525. doi: 10.3389/fmicb.2020.584525. eCollection 2020.
A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth.
Frontiers in microbiology
Carolin Schunke, Stefanie Pöggeler, Daniela Elisabeth Nordzieke
Affiliations
Affiliations
- Department of Genetics of Eukaryotic Microorganisms, Institute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
PMID: 33224121
PMCID: PMC7669831 DOI: 10.3389/fmicb.2020.584525
Abstract
Chemical gradients are surrounding living organisms in all habitats of life. Microorganisms, plants and animals have developed specific mechanisms to sense such gradients. Upon perception, chemical gradients can be categorized either as favorable, like nutrients or hormones, or as disadvantageous, resulting in a clear orientation toward the gradient and avoiding strategies, respectively. Being sessile organisms, fungi use chemical gradients for their orientation in the environment. Integration of this data enables them to successfully explore nutrient sources, identify probable plant or animal hosts, and to communicate during sexual reproduction or early colony development. We have developed a 3D printed device allowing a highly standardized, rapid and low-cost investigation of chemotropic growth processes in fungi. Since the 3D printed device is placed on a microscope slide, detailed microscopic investigations and documentation of the chemotropic process is possible. Using this device, we provide evidence that germlings derived from oval conidia of the hemibiotrophic plant pathogen
Copyright © 2020 Schunke, Pöggeler and Nordzieke.
Keywords: 3D printed device; Colletotrichum graminicola; chemotropism; filamentous fungi; glucose
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