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Abdillahi AM, Lee KW, McLuckey SA. Mass Analysis of Macro-molecular Analytes via Multiply-Charged Ion Attachment. Anal Chem. 2020;92(24):16301-16306doi: 10.1021/acs.analchem.0c04335.
Abdillahi, A. M., Lee, K. W., & McLuckey, S. A. (2020). Mass Analysis of Macro-molecular Analytes via Multiply-Charged Ion Attachment. Analytical chemistry, 92(24), 16301-16306. https://doi.org/10.1021/acs.analchem.0c04335
Abdillahi, Abdirahman M, et al. "Mass Analysis of Macro-molecular Analytes via Multiply-Charged Ion Attachment." Analytical chemistry vol. 92,24 (2020): 16301-16306. doi: https://doi.org/10.1021/acs.analchem.0c04335
Abdillahi AM, Lee KW, McLuckey SA. Mass Analysis of Macro-molecular Analytes via Multiply-Charged Ion Attachment. Anal Chem. 2020 Dec 15;92(24):16301-16306. doi: 10.1021/acs.analchem.0c04335. Epub 2020 Dec 04. PMID: 33275425; PMCID: PMC7738406.
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Anal Chem. 2020 Dec 15;92(24):16301-16306. doi: 10.1021/acs.analchem.0c04335. Epub 2020 Dec 04.

Mass Analysis of Macro-molecular Analytes via Multiply-Charged Ion Attachment.

Analytical chemistry

Abdirahman M Abdillahi, Kenneth W Lee, Scott A McLuckey

Affiliations

  1. Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.

PMID: 33275425 PMCID: PMC7738406 DOI: 10.1021/acs.analchem.0c04335

Abstract

A novel gas-phase charge and mass manipulation approach is demonstrated to facilitate the mass measurement of high mass complexes within the context of native mass spectrometry. Electrospray ionization applied to solutions generated under native or near-native conditions has been demonstrated to be capable of preserving biologically relevant complexes into the gas phase as multiply charged ions suitable for mass spectrometric analysis. However, charge state distributions tend to be narrow and extensive salt adduction, heterogeneity, and so on tend to lead to significantly broadened peaks. These issues can compromise mass measurement of high mass bio-complexes, particularly when charge states are not clearly resolved. In this work, we show that the attachment of high mass ions of known mass and charge to populations of ions of interest can lead to well-separated signals that can yield confident charge state and mass assignments from otherwise poorly resolved signals.

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