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Jones KS, Parkington DA, Cox LJ, et al. Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status. Ann N Y Acad Sci. 2020;1498(1):77-84doi: 10.1111/nyas.14547.
Jones, K. S., Parkington, D. A., Cox, L. J., & Koulman, A. (2021). Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status. Annals of the New York Academy of Sciences, 1498(1), 77-84. https://doi.org/10.1111/nyas.14547
Jones, Kerry S, et al. "Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status." Annals of the New York Academy of Sciences vol. 1498,1 (2021): 77-84. doi: https://doi.org/10.1111/nyas.14547
Jones KS, Parkington DA, Cox LJ, Koulman A. Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status. Ann N Y Acad Sci. 2021 Aug;1498(1):77-84. doi: 10.1111/nyas.14547. Epub 2020 Dec 22. PMID: 33354793; PMCID: PMC8451777.
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Ann N Y Acad Sci. 2021 Aug;1498(1):77-84. doi: 10.1111/nyas.14547. Epub 2020 Dec 22.

Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status.

Annals of the New York Academy of Sciences

Kerry S Jones, Damon A Parkington, Lorna J Cox, Albert Koulman

Affiliations

  1. NIHR BRC Nutritional Biomarker Laboratory, MRC Epidemiology Unit, University of Cambridge, Cambridge, UK.
  2. MRC Elsie Widdowson Laboratory, Cambridge, UK.

PMID: 33354793 PMCID: PMC8451777 DOI: 10.1111/nyas.14547

Abstract

Vitamin B1 (thiamine) is an essential nutrient that acts as a cofactor for a number of metabolic processes, particularly in energy metabolism. Symptoms of classic thiamine deficiency are recognized as beriberi, although clinical symptoms are nonspecific and recognition of subclinical deficiency is difficult. Therefore, reliable biomarkers of thiamine status are required. Thiamine diphosphate is a cofactor for transketolase, including erythrocyte transketolase (ETK). The ETK activity assay as an indirect, functional marker of thiamine status has been used for over 50 years. The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for deficiency, partly due to a lack of assay harmonization. Here, we provide a step-by-step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient, including detailed explanations of equipment and chemicals required and guidance for quality control procedures. Harmonization of the protocol will provide the basis for the development of internationally recognized cutoffs for thiamine insufficiency. The establishment of quality control materials and a quality assurance scheme are recommended to provide reliability. This will ensure that the ETK activity assay remains an important method for the assessment of thiamine status.

© 2020 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals, LLC on behalf of New York Academy of Sciences.

Keywords: ETKAC; beriberi; thiamine; vitamin B1

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