Bioprocess Biosyst Eng. 2021 Apr;44(4):737-747. doi: 10.1007/s00449-020-02482-7. Epub 2021 Jan 02.

Feeding strategies to optimize vanillin production by Amycolatopsis sp. ATCC 39116.

Bioprocess and biosystems engineering

Rita Valério, Ana R S Bernardino, Cristiana A V Torres, Carla Brazinha, Maria L Tavares, João G Crespo, Maria A M Reis

PMID: 33389106 DOI: 10.1007/s00449-020-02482-7

Abstract

The growing consumer demand for natural products led to an increasing interest in vanillin production by biotechnological routes. In this work, the biotechnological vanillin production by Amycolatopsis sp. ATCC 39116 is studied using ferulic acid as precursor, aiming to achieve maximized vanillin productivities. During biotech-vanillin production, the effects of glucose, vanillin and ferulic acid concentrations in the broth proved to be relevant for vanillin productivity. Concerning glucose, its presence in the broth during the production phase avoids vanillin conversion to vanillic acid and, consequently, increases vanillin production. To avoid the accumulation of vanillin up to a toxic concentration level, a multiple-pulse-feeding strategy is implemented, with intercalated vanillin removal from the broth and biomass recovery. This strategy turned out fruitful, leading to 0.46 g L

Keywords: Biotech vanillin; Ferulic acid; Pulse feeding strategy

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Publication Types

• Journal Article

Grant support

• SFRH/BD/138011/2018/Fundação para a Ciência e a Tecnologia
• PD/BDE/113543/2015/Fundação para a Ciência e a Tecnologia
• UID/Multi/04378/2019/Fundação para a Ciência e a Tecnologia
• UID/QUI/50006/2013/Fundação para a Ciência e a Tecnologia
• PD/BDE/113543/2015/COPAM
• POCI-01-0145-FEDER - 007265/European Regional Development Fund