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Rumpler É, Takács S, Göcz B, et al. Kisspeptin Neurons in the Infundibular Nucleus of Ovariectomized Cats and Dogs Exhibit Unique Anatomical and Neurochemical Characteristics. Front Neurosci. 2020;14:598707doi: 10.3389/fnins.2020.598707.
Rumpler, �. �., Takács, S., Göcz, B., Baska, F., Szenci, O., Horváth, A., Ciofi, P., Hrabovszky, E., & Skrapits, K. (2020). Kisspeptin Neurons in the Infundibular Nucleus of Ovariectomized Cats and Dogs Exhibit Unique Anatomical and Neurochemical Characteristics. Frontiers in neuroscience, 14598707. https://doi.org/10.3389/fnins.2020.598707
Rumpler, Éva, et al. "Kisspeptin Neurons in the Infundibular Nucleus of Ovariectomized Cats and Dogs Exhibit Unique Anatomical and Neurochemical Characteristics." Frontiers in neuroscience vol. 14 (2020): 598707. doi: https://doi.org/10.3389/fnins.2020.598707
Rumpler É, Takács S, Göcz B, Baska F, Szenci O, Horváth A, Ciofi P, Hrabovszky E, Skrapits K. Kisspeptin Neurons in the Infundibular Nucleus of Ovariectomized Cats and Dogs Exhibit Unique Anatomical and Neurochemical Characteristics. Front Neurosci. 2020 Dec 02;14:598707. doi: 10.3389/fnins.2020.598707. eCollection 2020. PMID: 33343288; PMCID: PMC7738562.
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Front Neurosci. 2020 Dec 02;14:598707. doi: 10.3389/fnins.2020.598707. eCollection 2020.

Kisspeptin Neurons in the Infundibular Nucleus of Ovariectomized Cats and Dogs Exhibit Unique Anatomical and Neurochemical Characteristics.

Frontiers in neuroscience

Éva Rumpler, Szabolcs Takács, Balázs Göcz, Ferenc Baska, Ottó Szenci, András Horváth, Philippe Ciofi, Erik Hrabovszky, Katalin Skrapits

Affiliations

  1. Laboratory of Reproductive Neurobiology, Institute of Experimental Medicine, Budapest, Hungary.
  2. Department of Exotic Animal and Wildlife Medicine, University of Veterinary Medicine, Budapest, Hungary.
  3. Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Üllõ, Hungary.
  4. MTA-SZIE Large Animal Clinical Research Group, University of Veterinary Medicine, Üllõ, Hungary.
  5. INSERM U1215, Neurocentre Magendie, University of Bordeaux, Bordeaux, France.

PMID: 33343288 PMCID: PMC7738562 DOI: 10.3389/fnins.2020.598707

Abstract

Neurons co-synthesizing kisspeptin (KP), neurokinin B (NKB), and dynorphin ("KNDy neurons") in the hypothalamic arcuate/infundibular nucleus (INF) form a crucial component of the gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) "pulse generator." The goal of our study was to characterize KP neuron distribution, neuropeptide phenotype and connectivity to GnRH cells in ovariectomized (OVX) dogs and cats with immunohistochemistry on formalin-fixed hypothalamic tissue sections. In both species, KP and NKB neurons occurred in the INF and the two cell populations overlapped substantially. Dynorphin was detected in large subsets of canine KP (56%) and NKB (37%) cells and feline KP (64%) and NKB (57%) cells; triple-labeled ("KNDy") somata formed ∼25% of all immunolabeled neurons. Substance P (SP) was present in 20% of KP and 29% of NKB neurons in OVX cats but not dogs, although 26% of KP and 24% of NKB neurons in a gonadally intact male dog also contained SP signal. Only in cats, cocaine- and amphetamine regulated transcript was also colocalized with KP (23%) and NKB (7%). In contrast with reports from mice, KP neurons did not express galanin in either carnivore. KP neurons innervated virtually all GnRH neurons in both species. Results of this anatomical study on OVX animals reveal species-specific features of canine and feline mediobasal hypothalamic KP neurons. Anatomical and neurochemical similarities to and differences from the homologous KP cells of more extensively studied rodent, domestic and primate species will enhance our understanding of obligate and facultative players in the molecular mechanisms underlying pulsatile GnRH/LH secretion.

Copyright © 2020 Rumpler, Takács, Göcz, Baska, Szenci, Horváth, Ciofi, Hrabovszky and Skrapits.

Keywords: KNDy neurons; arcuate nucleus; carnivores; hypothalamus; neurokinin B; neuropeptides; reproduction

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

  1. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2129-34 - PubMed
  2. Eur J Neurosci. 2010 Jun;31(11):1984-98 - PubMed
  3. Neuroendocrinology. 2004;80(4):264-72 - PubMed
  4. Front Endocrinol (Lausanne). 2017 Aug 07;8:191 - PubMed
  5. Endocrinology. 2008 Apr;149(4):1979-86 - PubMed
  6. Theriogenology. 2016 Jul 15;86(2):589-95 - PubMed
  7. J Histochem Cytochem. 1996 Dec;44(12):1353-62 - PubMed
  8. Endocrinology. 2005 Oct;146(10):4431-6 - PubMed
  9. J Comp Neurol. 2000 Nov 27;427(4):593-603 - PubMed
  10. Elife. 2016 Aug 23;5: - PubMed
  11. PLoS One. 2013 Aug 19;8(8):e72369 - PubMed
  12. Neuroendocrinology. 2020 Apr 16;: - PubMed
  13. Endocrinology. 2013 Aug;154(8):2750-60 - PubMed
  14. Front Endocrinol (Lausanne). 2011 Dec 01;2:80 - PubMed
  15. Cell Tissue Res. 1980;209(3):499-503 - PubMed
  16. J Neuroendocrinol. 2012 Mar;24(3):464-76 - PubMed
  17. N Engl J Med. 2003 Oct 23;349(17):1614-27 - PubMed
  18. Endocrinology. 2008 Aug;149(8):3926-32 - PubMed
  19. Front Neuroendocrinol. 2000 Jul;21(3):220-62 - PubMed
  20. J Neurosci. 1999 May 15;19(10):RC5 - PubMed
  21. Reprod Nutr Dev. 1980;20(5A):1489-501 - PubMed
  22. Neuroendocrinology. 2014;99(3-4):178-89 - PubMed
  23. Brain Res Rev. 2008 Mar;57(2):277-87 - PubMed
  24. Brain Res. 2010 Dec 10;1364:90-102 - PubMed
  25. Biochem Biophys Res Commun. 2004 Jul 23;320(2):383-8 - PubMed
  26. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1761-6 - PubMed
  27. Endocrinology. 2006 Feb;147(2):1007-13 - PubMed
  28. J Neurosci. 2009 Sep 23;29(38):11859-66 - PubMed
  29. Endocrinology. 2006 Dec;147(12):5817-25 - PubMed
  30. Endocrinology. 2012 Oct;153(10):4978-89 - PubMed
  31. Endocrinology. 2012 Nov;153(11):5428-39 - PubMed
  32. Science. 1978 Nov 10;202(4368):631-3 - PubMed
  33. Neuroendocrinology. 2016;103(6):711-23 - PubMed
  34. Front Neurosci. 2015 Feb 10;9:29 - PubMed
  35. Endocrinology. 2008 Sep;149(9):4387-95 - PubMed
  36. Front Endocrinol (Lausanne). 2013 Sep 20;4:130 - PubMed
  37. Endocrinology. 2011 Nov;152(11):4265-75 - PubMed
  38. Endocrinology. 2005 Jul;146(7):2976-84 - PubMed
  39. Brain Res Bull. 1990 Mar;24(3):395-9 - PubMed
  40. J Neurosci. 2005 Dec 7;25(49):11349-56 - PubMed
  41. Neuroendocrinology. 1975;18(4):290-8 - PubMed
  42. PLoS One. 2014 Aug 01;9(8):e103977 - PubMed
  43. J Neuroendocrinol. 2006 Jul;18(7):534-41 - PubMed
  44. Endocrinology. 2010 Aug;151(8):3479-89 - PubMed
  45. Neuroimage. 2007 Mar;35(1):9-15 - PubMed
  46. J Reprod Dev. 2019 Aug 9;65(4):335-343 - PubMed
  47. J Neuroendocrinol. 2017 Jun;29(6): - PubMed
  48. Neuroscience. 2006 Sep 15;141(4):1731-45 - PubMed
  49. Neurosci Lett. 2006 Jul 3;401(3):225-30 - PubMed
  50. Endocrinology. 2008 Aug;149(8):4151-7 - PubMed
  51. Nat Commun. 2013;4:2492 - PubMed
  52. J Neurosci. 2010 Feb 24;30(8):3124-32 - PubMed
  53. Endocrinology. 1999 Dec;140(12):5929-36 - PubMed
  54. J Reprod Dev. 2007 Apr;53(2):367-78 - PubMed
  55. Neuroendocrinology. 2011;94(4):323-32 - PubMed
  56. Endocrinology. 2004 Sep;145(9):4073-7 - PubMed
  57. Neuroendocrinology. 2019;109(3):230-241 - PubMed
  58. Endocrinology. 2010 Jan;151(1):301-11 - PubMed
  59. J Comp Neurol. 2006 Oct 10;498(5):712-26 - PubMed
  60. Endocrinology. 2018 Sep 1;159(9):3200-3208 - PubMed
  61. Nihon Juigaku Zasshi. 1985 Dec;47(6):931-42 - PubMed
  62. Endocrinology. 1993 Aug;133(2):896-903 - PubMed
  63. J Clin Endocrinol Metab. 2005 Dec;90(12):6609-15 - PubMed
  64. Brain Res Bull. 2000 Mar 1;51(4):281-91 - PubMed
  65. Endocrinology. 2012 Jun;153(6):2800-12 - PubMed
  66. Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):E10216-E10223 - PubMed
  67. Front Cell Neurosci. 2015 Sep 04;9:348 - PubMed
  68. Endocrinology. 2008 Nov;149(11):5770-82 - PubMed
  69. Endocrinology. 2005 Jan;146(1):156-63 - PubMed
  70. Peptides. 1984;5 Suppl 1:195-207 - PubMed
  71. Neurosci Lett. 1994 Dec 5;182(2):193-6 - PubMed
  72. Neuroscience. 2010 Mar 17;166(2):680-97 - PubMed
  73. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10972-6 - PubMed
  74. Endocrinology. 2013 Aug;154(8):2761-71 - PubMed
  75. Neuroendocrinology. 2014;100(2-3):141-52 - PubMed
  76. Endocrinology. 2007 Dec;148(12):5752-60 - PubMed
  77. Endocr Rev. 1986 Feb;7(1):11-23 - PubMed
  78. J Comp Neurol. 2011 Dec 1;519(17):3456-69 - PubMed
  79. Endocrinology. 2013 Aug;154(8):2821-32 - PubMed
  80. J Reprod Fertil Suppl. 1997;51:177-84 - PubMed
  81. J Neurosci. 2008 Aug 6;28(32):8003-13 - PubMed

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