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Siehler J, Blöchinger AK, Akgün M, et al. Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8). Stem Cell Res. 2020;50:102126doi: 10.1016/j.scr.2020.102126.
Siehler, J., Blöchinger, A. K., Akgün, M., Wang, X., Shahryari, A., Geerlof, A., Lickert, H., & Burtscher, I. (2020). Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8). Stem cell research, 50102126. https://doi.org/10.1016/j.scr.2020.102126
Siehler, Johanna, et al. "Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8)." Stem cell research vol. 50 (2020): 102126. doi: https://doi.org/10.1016/j.scr.2020.102126
Siehler J, Blöchinger AK, Akgün M, Wang X, Shahryari A, Geerlof A, Lickert H, Burtscher I. Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8). Stem Cell Res. 2020 Dec 16;50:102126. doi: 10.1016/j.scr.2020.102126. Epub 2020 Dec 16. PMID: 33373890.
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Stem Cell Res. 2020 Dec 16;50:102126. doi: 10.1016/j.scr.2020.102126. Epub 2020 Dec 16.

Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

Stem cell research

Johanna Siehler, Anna Karolina Blöchinger, Melis Akgün, Xianming Wang, Alireza Shahryari, Arie Geerlof, Heiko Lickert, Ingo Burtscher

Affiliations

  1. Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaninger Straße 22, 81675 München, Germany.
  2. Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaninger Straße 22, 81675 München, Germany; Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
  3. Institute of Structural Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
  4. Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaninger Straße 22, 81675 München, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany. Electronic address: [email protected].
  5. Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany. Electronic address: [email protected].

PMID: 33373890 DOI: 10.1016/j.scr.2020.102126

Abstract

The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.

Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

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