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Prabhakar N, Belevich I, Peurla M, et al. Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes. Nanomaterials (Basel). 2020;11(1)doi: 10.3390/nano11010014.
Prabhakar, N., Belevich, I., Peurla, M., Heiligenstein, X., Chang, H. C., Sahlgren, C., Jokitalo, E., & Rosenholm, J. M. (2020). Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes. Nanomaterials (Basel, Switzerland), 11(1), . https://doi.org/10.3390/nano11010014
Prabhakar, Neeraj, et al. "Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes." Nanomaterials (Basel, Switzerland) vol. 11,1 (2020). doi: https://doi.org/10.3390/nano11010014
Prabhakar N, Belevich I, Peurla M, Heiligenstein X, Chang HC, Sahlgren C, Jokitalo E, Rosenholm JM. Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes. Nanomaterials (Basel). 2020 Dec 23;11(1). doi: 10.3390/nano11010014. PMID: 33374705; PMCID: PMC7822478.
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Nanomaterials (Basel). 2020 Dec 23;11(1). doi: 10.3390/nano11010014.

Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes.

Nanomaterials (Basel, Switzerland)

Neeraj Prabhakar, Ilya Belevich, Markus Peurla, Xavier Heiligenstein, Huan-Cheng Chang, Cecilia Sahlgren, Eija Jokitalo, Jessica M Rosenholm

Affiliations

  1. Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland.
  2. Cell Biology, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland.
  3. Electron Microscopy Unit, Helsinki Institute of Life Science-Institute of Biotechnology, University of Helsinki, FI-00014 Helsinki, Finland.
  4. Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland.
  5. Cancer Research Laboratory FICAN West, Institute of Biomedicine, University of Turku, 20520 Turku, Finland.
  6. Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland.
  7. CryoCapCell, 155 Boulevard de l'Hopital, 75013 Paris, France.
  8. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.

PMID: 33374705 PMCID: PMC7822478 DOI: 10.3390/nano11010014

Abstract

Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.

Keywords: 3D CLEM; correlative microscopy; volume imaging

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