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Güzel C, van den Berg CB, Koopman S, et al. Cerebrospinal Fluid of Preeclamptic and Normotensive Pregnant Women Compared to Nonpregnant Women Analyzed with Mass Spectrometry. ACS Omega. 2020;5(50):32256-32266doi: 10.1021/acsomega.0c03910.
Güzel, C., van den Berg, C. B., Koopman, S., van Krugten, R. J., Stoop, M., Stingl, C., Duvekot, J. J., & Luider, T. M. (2020). Cerebrospinal Fluid of Preeclamptic and Normotensive Pregnant Women Compared to Nonpregnant Women Analyzed with Mass Spectrometry. ACS omega, 5(50), 32256-32266. https://doi.org/10.1021/acsomega.0c03910
Güzel, Coşkun, et al. "Cerebrospinal Fluid of Preeclamptic and Normotensive Pregnant Women Compared to Nonpregnant Women Analyzed with Mass Spectrometry." ACS omega vol. 5,50 (2020): 32256-32266. doi: https://doi.org/10.1021/acsomega.0c03910
Güzel C, van den Berg CB, Koopman S, van Krugten RJ, Stoop M, Stingl C, Duvekot JJ, Luider TM. Cerebrospinal Fluid of Preeclamptic and Normotensive Pregnant Women Compared to Nonpregnant Women Analyzed with Mass Spectrometry. ACS Omega. 2020 Dec 10;5(50):32256-32266. doi: 10.1021/acsomega.0c03910. eCollection 2020 Dec 22. PMID: 33376863; PMCID: PMC7758887.
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ACS Omega. 2020 Dec 10;5(50):32256-32266. doi: 10.1021/acsomega.0c03910. eCollection 2020 Dec 22.

Cerebrospinal Fluid of Preeclamptic and Normotensive Pregnant Women Compared to Nonpregnant Women Analyzed with Mass Spectrometry.

ACS omega

Coşkun Güzel, Caroline B van den Berg, Seppe Koopman, Robbert Jan van Krugten, Marcel Stoop, Christoph Stingl, Johannes J Duvekot, Theo M Luider

Affiliations

  1. Laboratory of Neuro-Oncology/Clinical & Cancer Proteomics, Department of Neurology, Erasmus University Medical Center, Room AE 312, Wytemaweg 80, Rotterdam 3015 CN, The Netherlands.
  2. Department of Obstetrics and Gynecology, Erasmus University Medical Center, Rotterdam 3015 GD, The Netherlands.
  3. Department of Anesthesiology, Maasstad Hospital, Rotterdam 3079 DZ, The Netherlands.

PMID: 33376863 PMCID: PMC7758887 DOI: 10.1021/acsomega.0c03910

Abstract

Preeclampsia is a pregnancy-specific multiorgan disorder in which impaired placental functioning and excessive oxidative stress play an important role. We previously showed distinct differences between cerebrospinal fluid proteins in patients with preeclampsia and normotensive pregnant women. An additional group of nonpregnant women was included to study the presence of pregnancy-related proteins in normotensive and preeclamptic pregnancies and whether pregnancy-related proteins were associated with preeclampsia. Cerebrospinal fluid samples were tryptically digested and subsequently measured with a nano-LC-tribrid Orbitrap mass spectrometry system. Proteins were identified by shotgun proteomic analysis based on a data-dependent acquisition method. Proteins identified in preeclampsia, normotensive pregnant controls, and nonpregnant groups were compared to the Progenesis method according to the criteria as previously described and with a secondary analysis using a Scaffold method including Benjamini-Hochberg correction for multiple testing. For preeclampsia, the Progenesis and the Scaffold method together identified 15 (eight proteins for both analyses with one overlap) proteins that were significantly different compared to normotensive control pregnancies. Three of these 15 proteins, which were elevated in cerebrospinal fluid of preeclamptic women, were described to be pregnancy proteins with a calcium-binding function. Using two analysis methods (Progenesis and Scaffold), four out of 15 differential proteins were associated with pregnancy, as described in the literature. Three out of the four pregnancy-related proteins were elevated in preeclampsia. Furthermore, the contribution of elevated (

© 2020 American Chemical Society.

Conflict of interest statement

The authors declare no competing financial interest.

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