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Liu J, Xue Z, Vann KR, et al. Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR Protoc. 2020;1(3):100155doi: 10.1016/j.xpro.2020.100155.
Liu, J., Xue, Z., Vann, K. R., Shi, X., & Kutateladze, T. G. (2020). Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR protocols, 1(3), 100155. https://doi.org/10.1016/j.xpro.2020.100155
Liu, Jiuyang, et al. "Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2." STAR protocols vol. 1,3 (2020): 100155. doi: https://doi.org/10.1016/j.xpro.2020.100155
Liu J, Xue Z, Vann KR, Shi X, Kutateladze TG. Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR Protoc. 2020 Oct 28;1(3):100155. doi: 10.1016/j.xpro.2020.100155. eCollection 2020 Dec 18. PMID: 33377049; PMCID: PMC7757301.
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STAR Protoc. 2020 Oct 28;1(3):100155. doi: 10.1016/j.xpro.2020.100155. eCollection 2020 Dec 18.

Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2.

STAR protocols

Jiuyang Liu, Zhaoyu Xue, Kendra R Vann, Xiaobing Shi, Tatiana G Kutateladze

Affiliations

  1. Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
  2. Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.

PMID: 33377049 PMCID: PMC7757301 DOI: 10.1016/j.xpro.2020.100155

Abstract

Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HERC2

© 2020 The Author(s).

Conflict of interest statement

The authors declare no competing interests.

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