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Rincon Fernandez Pacheco D, Sabet S, Breunig JJ. Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR). STAR Protoc. 2020;1(3):100199doi: 10.1016/j.xpro.2020.100199.
Rincon Fernandez Pacheco, D., Sabet, S., & Breunig, J. J. (2020). Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR). STAR protocols, 1(3), 100199. https://doi.org/10.1016/j.xpro.2020.100199
Rincon Fernandez Pacheco, David, et al. "Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR)." STAR protocols vol. 1,3 (2020): 100199. doi: https://doi.org/10.1016/j.xpro.2020.100199
Rincon Fernandez Pacheco D, Sabet S, Breunig JJ. Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR). STAR Protoc. 2020 Dec 09;1(3):100199. doi: 10.1016/j.xpro.2020.100199. eCollection 2020 Dec 18. PMID: 33377093; PMCID: PMC7757563.
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STAR Protoc. 2020 Dec 09;1(3):100199. doi: 10.1016/j.xpro.2020.100199. eCollection 2020 Dec 18.

Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR).

STAR protocols

David Rincon Fernandez Pacheco, Sara Sabet, Joshua J Breunig

Affiliations

  1. Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  2. Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  3. Center for Neural Sciences in Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  4. Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  5. Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.

PMID: 33377093 PMCID: PMC7757563 DOI: 10.1016/j.xpro.2020.100199

Abstract

This protocol focuses on the cloning and stable integration of sequences of interest by the use of a mosaic analysis with dual recombinases (MADR) plasmid that includes fusion proteins or independent proteins under the control of 2A peptide or IRES elements. Additionally, we describe how to generate a neural stem cell culture from Gt(ROSA)26Sort

© 2020 The Author(s).

Keywords: CRISPR; Gene Expression; Model Organisms; Molecular Biology; Neuroscience

Conflict of interest statement

Cedars-Sinai has filed for patent protection for in vivo dual recombinase-mediated cassette exchange and disease models thereof.

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