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Urban-Chmiel R, Balicki I, Świąder K, et al. The in vitro efficacy of eye drops containing a bacteriophage solution specific for Staphylococcus spp. isolated from dogs with bacterial conjunctivitis. Ir Vet J. 2020;73(1):21doi: 10.1186/s13620-020-00175-x.
Urban-Chmiel, R., Balicki, I., Świąder, K., Nowaczek, A., Pyzik, E., Stępień-Pyśniak, D., Marek, A., Puchalski, A., Wernicki, A., Poleszak, E., & Dec, M. (2020). The in vitro efficacy of eye drops containing a bacteriophage solution specific for Staphylococcus spp. isolated from dogs with bacterial conjunctivitis. Irish veterinary journal, 73(1), 21. https://doi.org/10.1186/s13620-020-00175-x
Urban-Chmiel, Renata, et al. "The in vitro efficacy of eye drops containing a bacteriophage solution specific for Staphylococcus spp. isolated from dogs with bacterial conjunctivitis." Irish veterinary journal vol. 73,1 (2020): 21. doi: https://doi.org/10.1186/s13620-020-00175-x
Urban-Chmiel R, Balicki I, Świąder K, Nowaczek A, Pyzik E, Stępień-Pyśniak D, Marek A, Puchalski A, Wernicki A, Poleszak E, Dec M. The in vitro efficacy of eye drops containing a bacteriophage solution specific for Staphylococcus spp. isolated from dogs with bacterial conjunctivitis. Ir Vet J. 2020 Nov 04;73(1):21. doi: 10.1186/s13620-020-00175-x. PMID: 33319700; PMCID: PMC7643432.
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Ir Vet J. 2020 Nov 04;73(1):21. doi: 10.1186/s13620-020-00175-x.

The in vitro efficacy of eye drops containing a bacteriophage solution specific for Staphylococcus spp. isolated from dogs with bacterial conjunctivitis.

Irish veterinary journal

Renata Urban-Chmiel, Ireneusz Balicki, Katarzyna Świąder, Anna Nowaczek, Ewelina Pyzik, Dagmara Stępień-Pyśniak, Agnieszka Marek, Andrzej Puchalski, Andrzej Wernicki, Ewa Poleszak, Marta Dec

Affiliations

  1. SubDepartment of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, Lublin, Poland.
  2. Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, Lublin, Poland.
  3. Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
  4. SubDepartment of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, Lublin, Poland. [email protected].

PMID: 33319700 PMCID: PMC7643432 DOI: 10.1186/s13620-020-00175-x

Abstract

BACKGROUND: The purpose of the study was to evaluate the in vitro antibacterial effect of experimental eye drops with bacteriophages in elimination of Staphylococcus spp. isolated from dogs with bacterial conjunctivitis.. The bacterial material was collected from dogs with independent clinical signs of bacterial conjunctivitis. Staphylococcus spp. were identified by phenotypic and genotypic methods (MALDI-TOF MS mass spectrometry). Antibiotic resistance was determined by the disc-diffusion method. Phage activity (Plaque forming units, PFU) was determined on double-layer agar plates. Phages with lytic titres > 10

RESULTS: The tests confirmed the occurrence of Staphylococcus spp. strains as etiological agents of bacterial conjunctivitis in dogs. A high percentage of strains were resistant to more than three antibiotics. The experimental phage eye drops used in the study exhibited 100% efficacy in vitro against the tested Staphylococcus isolates. Particularly noteworthy is the long duration of activity and constant antibacterial lytic titre of ≥10

CONCLUSIONS: The results represent the first stage of research and require continuation in vivo. If positive effects are obtained in animals, the results can be used in applied research in humans and animals.

Keywords: Antibiotic resistance; Bacterial infections; Conjunctival diseases; Experimental medicine; Ophthalmology

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