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Islam H, Amato A, Bonafiglia JT, et al. Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle. Pflugers Arch. 2021;473(2):241-252doi: 10.1007/s00424-020-02499-7.
Islam, H., Amato, A., Bonafiglia, J. T., Rahman, F. A., Preobrazenski, N., Ma, A., Simpson, C. A., Quadrilatero, J., & Gurd, B. J. (2021). Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle. Pflugers Archiv : European journal of physiology, 473(2), 241-252. https://doi.org/10.1007/s00424-020-02499-7
Islam, Hashim, et al. "Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle." Pflugers Archiv : European journal of physiology vol. 473,2 (2021): 241-252. doi: https://doi.org/10.1007/s00424-020-02499-7
Islam H, Amato A, Bonafiglia JT, Rahman FA, Preobrazenski N, Ma A, Simpson CA, Quadrilatero J, Gurd BJ. Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle. Pflugers Arch. 2021 Feb;473(2):241-252. doi: 10.1007/s00424-020-02499-7. Epub 2021 Jan 08. PMID: 33420549.
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Pflugers Arch. 2021 Feb;473(2):241-252. doi: 10.1007/s00424-020-02499-7. Epub 2021 Jan 08.

Increasing whole-body energetic stress does not augment fasting-induced changes in human skeletal muscle.

Pflugers Archiv : European journal of physiology

Hashim Islam, Alessandra Amato, Jacob T Bonafiglia, Fasih A Rahman, Nicholas Preobrazenski, Andrew Ma, Craig A Simpson, Joe Quadrilatero, Brendon J Gurd

Affiliations

  1. School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
  2. Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.
  3. School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada. [email protected].

PMID: 33420549 DOI: 10.1007/s00424-020-02499-7

Abstract

Fasting rapidly (≤ 6 h) activates mitochondrial biogenic pathways in rodent muscle, an effect that is absent in human muscle following prolonged (10-72 h) fasting. We tested the hypotheses that fasting-induced changes in human muscle occur shortly after food withdrawal and are modulated by whole-body energetic stress. Vastus lateralis biopsies were obtained from ten healthy males before, during (4 h), and after (8 h) two supervised fasts performed with (FAST+EX) or without (FAST) 2 h of arm ergometer exercise (~ 400 kcal of added energy expenditure). PGC-1α mRNA (primary outcome measure) was non-significantly reduced (p = 0.065 [η

Keywords: AMPK; Caloric restriction; Food deprivation; Metabolic flexibility; PGC-1α; SIRT1

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