Display options
Cite
Jäkel H, Scheinpflug K, Mühldorfer K, et al. In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C. J Anim Sci Biotechnol. 2021;12(1):9doi: 10.1186/s40104-020-00530-6.
Jäkel, H., Scheinpflug, K., Mühldorfer, K., Gianluppi, R., Lucca, M. S., Mellagi, A. P. G., Bortolozzo, F. P., & Waberski, D. (2021). In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C. Journal of animal science and biotechnology, 12(1), 9. https://doi.org/10.1186/s40104-020-00530-6
Jäkel, Helen, et al. "In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C." Journal of animal science and biotechnology vol. 12,1 (2021): 9. doi: https://doi.org/10.1186/s40104-020-00530-6
Jäkel H, Scheinpflug K, Mühldorfer K, Gianluppi R, Lucca MS, Mellagi APG, Bortolozzo FP, Waberski D. In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C. J Anim Sci Biotechnol. 2021 Jan 11;12(1):9. doi: 10.1186/s40104-020-00530-6. PMID: 33423688; PMCID: PMC7798330.
Copy Download .nbib Format: NLM
Share it on

J Anim Sci Biotechnol. 2021 Jan 11;12(1):9. doi: 10.1186/s40104-020-00530-6.

In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C.

Journal of animal science and biotechnology

Helen Jäkel, Kathi Scheinpflug, Kristin Mühldorfer, Rafael Gianluppi, Matheus Schardong Lucca, Ana Paula Gonçalves Mellagi, Fernando Pandolfo Bortolozzo, Dagmar Waberski

Affiliations

  1. Unit of Reproductive Medicine of the Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Bünteweg 15, 30559, Hannover, Germany.
  2. Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.
  3. Animal Science Department, Swine Sector, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves, 9090, Porto Alegre, 91540-000, Brazil.
  4. Unit of Reproductive Medicine of the Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Bünteweg 15, 30559, Hannover, Germany. [email protected].

PMID: 33423688 PMCID: PMC7798330 DOI: 10.1186/s40104-020-00530-6

Abstract

BACKGROUND: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility.

OBJECTIVES: To examine chilling injury and the number and type of bacteria in boar semen stored at 5 °C in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions.

MATERIAL AND METHODS: Boar ejaculates were extended with AndroStar® Premium, stored at 17 °C with and at 5 °C without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study.

RESULTS: Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher (P < 0.05) up until 72 h in sperm stored at 5 °C compared to 17 °C but did not differ after 144 h. After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 °C, bacteria counts were below 10

CONCLUSION: Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.

Keywords: Antibiotics; Bacteria; Boar semen; Chilling; Fertility; Semen preservation

References

  1. Theriogenology. 2013 Oct 1;80(6):565-70 - PubMed
  2. Trends Ecol Evol. 2020 Mar;35(3):220-234 - PubMed
  3. Zentralbl Veterinarmed A. 1994 Oct;41(8):640-4 - PubMed
  4. Anim Reprod Sci. 2020 Sep;220:106284 - PubMed
  5. Reprod Fertil Dev. 2014;26(6):883-97 - PubMed
  6. Cytometry A. 2011 May;79(5):338-48 - PubMed
  7. Theriogenology. 2016 Jul 1;86(1):366-72 - PubMed
  8. Cryobiology. 2013 Feb;66(1):69-75 - PubMed
  9. Cryobiology. 1992 Apr;29(2):255-66 - PubMed
  10. Anim Reprod Sci. 2011 Sep;127(3-4):176-82 - PubMed
  11. Theriogenology. 2016 Jan 1;85(1):21-6 - PubMed
  12. Theriogenology. 2019 Oct 1;137:2-7 - PubMed
  13. Theriogenology. 2016 May;85(8):1421-31 - PubMed
  14. Theriogenology. 2019 Oct 1;137:104-112 - PubMed
  15. Anim Reprod Sci. 2012 May;132(1-2):66-73 - PubMed
  16. PLoS One. 2020 Jun 9;15(6):e0234339 - PubMed
  17. Annu Rev Anim Biosci. 2018 Feb 15;6:255-286 - PubMed
  18. Theriogenology. 1994 Oct;42(5):831-40 - PubMed
  19. Theriogenology. 2000 Mar 15;53(5):1167-76 - PubMed
  20. Andrology. 2013 May;1(3):376-86 - PubMed
  21. Anim Reprod Sci. 2010 Jul;120(1-4):95-104 - PubMed
  22. Theriogenology. 2015 Feb;83(3):430-7 - PubMed
  23. Theriogenology. 2019 Apr 15;129:37-45 - PubMed
  24. Theriogenology. 1997 Jul 15;48(2):209-19 - PubMed
  25. Vet Microbiol. 2011 Apr 21;149(1-2):163-71 - PubMed
  26. Apoptosis. 2007 May;12(5):841-55 - PubMed
  27. Infect Drug Resist. 2018 Oct 10;11:1645-1658 - PubMed
  28. Theriogenology. 2020 Jun;149:131-138 - PubMed
  29. Theriogenology. 2005 Jan 15;63(2):370-82 - PubMed
  30. Reprod Domest Anim. 2011 Sep;46 Suppl 2:45-8 - PubMed
  31. Anim Reprod Sci. 2014 Jul;148(1-2):72-82 - PubMed
  32. J Anim Sci. 2004 Oct;82(10):3058-70 - PubMed
  33. Reproduction. 2001 Dec;122(6):889-98 - PubMed
  34. J Dairy Sci. 2019 Dec;102(12):11652-11669 - PubMed
  35. Anim Reprod Sci. 2017 Feb;177:65-78 - PubMed
  36. Sci Rep. 2019 Oct 14;9(1):14748 - PubMed

Publication Types

Grant support