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Yaghmour MH, Thiele C, Kuerschner L. An advanced method for propargylcholine phospholipid detection by direct-infusion MS. J Lipid Res. 2021;62:100022doi: 10.1016/j.jlr.2021.100022.
Yaghmour, M. H., Thiele, C., & Kuerschner, L. (2021). An advanced method for propargylcholine phospholipid detection by direct-infusion MS. Journal of lipid research, 62100022. https://doi.org/10.1016/j.jlr.2021.100022
Yaghmour, Mohamed H, et al. "An advanced method for propargylcholine phospholipid detection by direct-infusion MS." Journal of lipid research vol. 62 (2021): 100022. doi: https://doi.org/10.1016/j.jlr.2021.100022
Yaghmour MH, Thiele C, Kuerschner L. An advanced method for propargylcholine phospholipid detection by direct-infusion MS. J Lipid Res. 2021 Jan 13;62:100022. doi: 10.1016/j.jlr.2021.100022. Epub 2021 Jan 13. PMID: 33453218; PMCID: PMC7900581.
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J Lipid Res. 2021 Jan 13;62:100022. doi: 10.1016/j.jlr.2021.100022. Epub 2021 Jan 13.

An advanced method for propargylcholine phospholipid detection by direct-infusion MS.

Journal of lipid research

Mohamed H Yaghmour, Christoph Thiele, Lars Kuerschner

Affiliations

  1. LIMES Life and Medical Sciences Institute, University of Bonn, Bonn, Germany.
  2. LIMES Life and Medical Sciences Institute, University of Bonn, Bonn, Germany. Electronic address: [email protected].

PMID: 33453218 PMCID: PMC7900581 DOI: 10.1016/j.jlr.2021.100022

Abstract

Phospholipids with a choline head group are an abundant component of cellular membranes and are involved in many important biological functions. For studies on the cell biology and metabolism of these lipids, traceable analogues where propargylcholine replaces the choline head group have proven useful. We present a novel method to analyze propargylcholine phospholipids by MS. The routine employs 1-radyl-2-lyso-sn-glycero-3-phosphopropargylcholines as labeled lysophosphatidylcholine precursors, which upon cellular conversion direct the traceable tag with superb specificity and efficiency to the primary target lipid class. Using azidopalmitate as a click-chemistry reporter, we introduce a highly specific, sensitive, and robust MS detection procedure for the propargylcholine phospholipids. In a first study, we apply the new technique to investigate choline phospholipid metabolism in brain endothelial cells. These experiments reveal differences in the metabolism of phosphatidylcholine and its pendant, ether phosphatidylcholine. The novel method described here opens a new, quantitative, and detailed view on propargylcholine phospholipid metabolism and will greatly facilitate future studies on choline phospholipid metabolism.

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Keywords: click; ether lipid; lipidomics; lysophosphatidylcholine; plasmalogen; propargyl-PC

Conflict of interest statement

Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.

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