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Alemnew B, Hoff ST, Abebe T, et al. Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection. BMC Immunol. 2021;22(1):9doi: 10.1186/s12865-021-00400-4.
Alemnew, B., Hoff, S. T., Abebe, T., Abebe, M., Aseffa, A., Howe, R., & Wassie, L. (2021). Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection. BMC immunology, 22(1), 9. https://doi.org/10.1186/s12865-021-00400-4
Alemnew, Birhan, et al. "Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection." BMC immunology vol. 22,1 (2021): 9. doi: https://doi.org/10.1186/s12865-021-00400-4
Alemnew B, Hoff ST, Abebe T, Abebe M, Aseffa A, Howe R, Wassie L. Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection. BMC Immunol. 2021 Jan 28;22(1):9. doi: 10.1186/s12865-021-00400-4. PMID: 33509080; PMCID: PMC7842038.
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BMC Immunol. 2021 Jan 28;22(1):9. doi: 10.1186/s12865-021-00400-4.

Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection.

BMC immunology

Birhan Alemnew, Soren T Hoff, Tamrat Abebe, Markos Abebe, Abraham Aseffa, Rawleigh Howe, Liya Wassie

Affiliations

  1. College of Health Sciences, Department of Medical Laboratory Sciences, Woldia University, Woldia, Ethiopia.
  2. Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  3. Statens Serum Institute, Copenhagen, Denmark.
  4. Department of Microbiology, Immunology and Parasitology, College of Health Sciences, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
  5. Armauer Hansen Research Institute, Addis Ababa, Ethiopia. [email protected].

PMID: 33509080 PMCID: PMC7842038 DOI: 10.1186/s12865-021-00400-4

Abstract

BACKGROUND: Understanding immune mechanisms, particularly the role of innate immune markers during latent TB infection remains elusive. The main objective of this study was to evaluate mRNA gene expression patterns of toll-like receptors (TLRs) as correlates of immunity during latent TB infection and further infer their roles as potential diagnostic biomarkers.

METHODS: Messenger RNA (mRNA) levels were analysed in a total of 64 samples collected from apparently healthy children and adolescents latently infected with tuberculosis (n = 32) or non-infected (n = 32). Relative expression in peripheral blood of selected genes encoding TLRs (TLR-1, TLR-2, TLR-4, TLR-6 and TLR-9) was determined with a quantitative real-time polymerase chain reaction (qRT-PCR) using specific primers and florescent labelled probes and a comparative threshold cycle method to define fold change. Data were analysed using Graph-Pad Prism 7.01 for Windows and a p-value less than 0.05 was considered statistically significant.

RESULTS: An increased mean fold change in the relative expression of TLR-2 and TLR-6 mRNA was observed in LTBI groups relative to non-LTBI groups (p < 0.05), whereas a slight fold decrease was observed for TLR-1 gene.

CONCLUSIONS: An increased mRNA expression of TLR-2 and TLR-6 was observed in latently infected individuals relative to those non-infected, possibly indicating the roles these biomarkers play in sustenance of the steady state interaction between the dormant TB bacilli and host immunity.

Keywords: Children; Latency; TLR; Tuberculosis; mRNA

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