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Weimann A, Plomgaard P, Hilsted LM, et al. Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry. Scand J Clin Lab Invest. 2021;81(2):127-136doi: 10.1080/00365513.2020.1871504.
Weimann, A., Plomgaard, P., Hilsted, L. M., Poulsen, H. E., & Larsen, E. L. (2021). Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry. Scandinavian journal of clinical and laboratory investigation, 81(2), 127-136. https://doi.org/10.1080/00365513.2020.1871504
Weimann, Allan, et al. "Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry." Scandinavian journal of clinical and laboratory investigation vol. 81,2 (2021): 127-136. doi: https://doi.org/10.1080/00365513.2020.1871504
Weimann A, Plomgaard P, Hilsted LM, Poulsen HE, Larsen EL. Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry. Scand J Clin Lab Invest. 2021 Apr;81(2):127-136. doi: 10.1080/00365513.2020.1871504. Epub 2021 Jan 19. PMID: 33461365.
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Scand J Clin Lab Invest. 2021 Apr;81(2):127-136. doi: 10.1080/00365513.2020.1871504. Epub 2021 Jan 19.

Quantification of biotin in plasma samples by column switching liquid chromatography - tandem mass spectrometry.

Scandinavian journal of clinical and laboratory investigation

Allan Weimann, Peter Plomgaard, Linda M Hilsted, Henrik E Poulsen, Emil L Larsen

Affiliations

  1. Department of Clinical Pharmacology, Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark.
  2. Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
  3. Faculty of Medicine, University of Copenhagen, Copenhagen, Denmark.

PMID: 33461365 DOI: 10.1080/00365513.2020.1871504

Abstract

Biotin (or Vitamin B7) is a vitamin where deficiency can be caused by inadequate intake. Biotin deficiency is rare, as most people get enough biotin from diet, since many foods contain biotin. In addition to biotin from food, intestinal bacteria can synthesize biotin, which can then be absorbed by the body. Supplementation with biotin has been advocated, mainly due to proposed beneficial effects on skin, nail and hair growth. There is no evidence that high biotin intakes are toxic, but a high intake may interfere with diagnostic assays that use biotin-streptavidin technology. These tests are commonly used to measure plasma concentrations of a wide range of hormones. Erroneous results may lead to misdiagnosis of various endocrine disorders. Supplementation with high-dose biotin has been used experimental for the treatment of diseases (e.g. multiple sclerosis) and high doses are used to obtain effect on nail and hair growth. On this background a demand for tests to determine if there is a risk of obtaining false test results when using biotin-streptavidin based tests have appeared. In this paper we present a method based on column switching liquid chromatography tandem mass spectrometry for the quantification of biotin in plasma and serum and explore the effects of biotin on an immunoassay based on biotin strept(avidin) chemistry.

Keywords: Biotin; streptavidin; tandem mass spectrometry; thyrotropin; triiodothyronine

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