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Deng Z, Hu X, Alahdal M, et al. High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process. PeerJ. 2021;9:e10656doi: 10.7717/peerj.10656.
Deng, Z., Hu, X., Alahdal, M., Liu, J., Zhao, Z., Chen, X., Xie, J., Duan, L., Wang, D., & Li, W. (2021). High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process. PeerJ, 9e10656. https://doi.org/10.7717/peerj.10656
Deng, Zhiqin, et al. "High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process." PeerJ vol. 9 (2021): e10656. doi: https://doi.org/10.7717/peerj.10656
Deng Z, Hu X, Alahdal M, Liu J, Zhao Z, Chen X, Xie J, Duan L, Wang D, Li W. High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process. PeerJ. 2021 Jan 15;9:e10656. doi: 10.7717/peerj.10656. eCollection 2021. PMID: 33520453; PMCID: PMC7812924.
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PeerJ. 2021 Jan 15;9:e10656. doi: 10.7717/peerj.10656. eCollection 2021.

High expression of MAPK-14 promoting the death of chondrocytes is an important signal of osteoarthritis process.

PeerJ

Zhiqin Deng, Xiaotian Hu, Murad Alahdal, Jianquan Liu, Zhe Zhao, Xiaoqiang Chen, Junxiong Xie, Li Duan, Daping Wang, Wencui Li

Affiliations

  1. Hand and Foot Surgery Department, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, Guangdong, China.
  2. Anhui Medical University, Hefei, China.

PMID: 33520453 PMCID: PMC7812924 DOI: 10.7717/peerj.10656

Abstract

BACKGROUND: Osteoarthritis (OA) is one of the most common degenerative diseases worldwide. Many researchers are studying the pathogenesis of OA, however, it is still unclear.

METHODS: Screening and validation of OA relevant hub genes are an important part of exploring their potential molecular mechanism. Therefore, this study aims to explore and verify the mechanisms of hub genes in the OA by bioinformatics, qPCR, fluorescence and propidium iodide staining.

RESULTS: Microarray datasets GSE43923, GSE55457 and GSE12021 were collected in the Gene Expression Omnibus (GEO), including 45 samples, which divided into 23 osteoarthritis knee joint samples and 22 samples of normal knee joint. Thereafter, 265 differentiallyexpressedgenes (DEGs) were identified in all, which divided into 199 upregulated genes and 66 downregulated genes. The hub genes MAPK-14, PTPRC, PTPN12 were upregulated, while B9D1 was downregulated. In order to further confirm the expression of screening differential genes in human chondrocytes, the human chondrocytes were extracted from a joint replacement surgery and stained with toluidine blue for identification. Compared with normal chondrocytes, OA chondrocytes had high expression of COL I protein and low expression of COL II protein. The expression levels of MAPK-14, PTPRC and PTPN12 in OA chondrocytes were significantly higher than the expression levels of B9D1 in normal chondrocytes. Moreover, the inflammatory necrosis of OA chondrocytes was increased compared with the normal chondrocytes by propidium iodide staining.

CONCLUSIONS: The high expression of MAPK-14 works as a promoter of chondrocytes death and an important signal of the osteoarthritis process.

©2021 Deng et al.

Keywords: Chondrocytes; Differentially expressed genes; Gene Ontology; MAPK-14; Osteoarthritis; Protein-protein interaction

Conflict of interest statement

The authors declare there are no competing interests.

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