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Almeida LC, Frade T, Correia RD, et al. Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces. Sci Rep. 2021;11(1):2237doi: 10.1038/s41598-021-81816-1.
Almeida, L. C., Frade, T., Correia, R. D., Niu, Y., Jin, G., Correia, J. P., & Viana, A. S. (2021). Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces. Scientific reports, 11(1), 2237. https://doi.org/10.1038/s41598-021-81816-1
Almeida, Luís C, et al. "Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces." Scientific reports vol. 11,1 (2021): 2237. doi: https://doi.org/10.1038/s41598-021-81816-1
Almeida LC, Frade T, Correia RD, Niu Y, Jin G, Correia JP, Viana AS. Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces. Sci Rep. 2021 Jan 26;11(1):2237. doi: 10.1038/s41598-021-81816-1. PMID: 33500469; PMCID: PMC7838280.
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Sci Rep. 2021 Jan 26;11(1):2237. doi: 10.1038/s41598-021-81816-1.

Electrosynthesis of polydopamine-ethanolamine films for the development of immunosensing interfaces.

Scientific reports

Luís C Almeida, Tânia Frade, Rui D Correia, Yu Niu, Gang Jin, Jorge P Correia, Ana S Viana

Affiliations

  1. Centro de Química Estrutural, Faculdade de Ciências da Universidade de Lisboa, 1749-016, Campo Grande, Lisbon, Portugal.
  2. NML, Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100190, China.
  3. Centro de Química Estrutural, Faculdade de Ciências da Universidade de Lisboa, 1749-016, Campo Grande, Lisbon, Portugal. [email protected].

PMID: 33500469 PMCID: PMC7838280 DOI: 10.1038/s41598-021-81816-1

Abstract

We report a straightforward and reproducible electrochemical approach to develop polydopamine-ethanolamine (ePDA-ETA) films to be used as immunosensing interfaces. ETA is strongly attached to polydopamine films during the potentiodynamic electropolymerization of dopamine. The great advantage of the electrochemical methods is to generate the oxidized species (quinones), which can readily react with ETA amine groups present in solution, with the subsequent incorporation of this molecule in the polymer. The presence of ETA and its effect on the electrosynthesis of polydopamine was accessed by cyclic voltammetry, ellipsometry, atomic force microscopy, FTIR and X-ray photoelectron spectroscopy. The adhesive and biocompatible films enable a facile protein linkage, are resilient to flow assays, and display intrinsic anti-fouling properties to block non-specific protein interactions, as monitored by real-time surface plasmon resonance, and confirmed by ellipsometry. Immunoglobulin G (IgG) and Anti-IgG were used in this work as model proteins for the affinity sensor. By using the one-step methodology (ePDA-ETA), the lower amount of immobilized biorecognition element, IgG, compared to that deposited on ePDA or on ETA post-modified film (ePDA/ETA), allied to the presence of ETA, improved the antibody-antigen affinity interaction. The great potential of the developed platform is its versatility to be used with any target biorecognition molecules, allowing both optical and electrochemical detection.

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